Sigma-Aldrich Introduces MISSION(R) LentiPlex(TM) Pooled shRNA Libraries to Enable Rapid Whole Genome Screening in Primary Cells, Stem Cells or Tumor Cells
The MISSION LentiPlex Pooled shRNA Libraries were developed from the entire TRC-1 human and mouse collections of more than 158,000 shRNA constructs targeting approximately 16,000 human genes and approximately 16,000 mouse genes. Combining Sigma-Aldrich’s high-throughput lentiviral production methodologies with the pooled library format enables researchers to perform genome-wide RNAi screens on various cell types, rapidly and efficiently discovering new targets without a large investment in infrastructure.
“Sigma-Aldrich is committed to providing customers powerful and unique screening formats that fully leverage the content of TRC,” said
LentiPlex enables researchers to screen for novel phenotypes in relevant cell lines and study gene function and disease, both in standard cell lines and difficult cell types such as primary, non-dividing, growth-arrested or terminally differentiated cells that are typically resistant to standard siRNA transfection or other viral delivery systems. In addition, Sigma-Aldrich’s shRNA libraries include a U6 promoter for shRNA expression in a wide variety of cell lines including embryonic stem cells. These characteristics mean researchers can now conduct high quality pooled shRNA screens in the cell lines of their choice.
The MISSION LentiPlex Pooled shRNA Libraries add to Sigma-Aldrich’s suite of MISSION shRNA products, which include the MISSION TRC-1 shRNA human (MISSION TRC-Hs1.0) and murine (MISSION TRC-Mm1.0) collections of whole genome arrayed libraries, gene family sets or individually prepared clones in glycerol, purified DNA or lentiviral formats. For more information on the MISSION LentiPlex Pooled shRNA Library, please visit http://www.sigma-aldrich.com/lentiplex.
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