Reprogramming Stem Cells to a More Basic Form Results in More Effective Transplant, Study Shows

November 3, 2011

Chinese stem cell scientists have published new research that improves the survival and effectiveness of transplanted stem cells. The research led by Dr Hsiao Chang Chan, from the Chinese University of Hong Kong, is published in STEM CELLS.

Durham, NC (PRWEB) November 03, 2011

Chinese stem cell scientists have published new research that improves the survival and effectiveness of transplanted stem cells. The research led by Dr Hsiao Chang Chan, from the Chinese University of Hong Kong, is published in STEM CELLS.

Research into differentiation has led to a variety of breakthroughs as stem cell researchers harvest cells from one part of the body and genetically adapt them to fulfill a specialized role. However, if the implanted cells are too much like the cells of the targeted area they may not have the plasticity to engraft and repair the injured tissue.

âœStem cell differentiation and transplantation has been shown to improve function in conditions including degenerative diseases and blood supply disorders,â said Dr Chan. âœHowever, the survival rate of transplanted cells in patients limits their overall effectiveness, which is a barrier to clinical use.â

To overcome this issue Dr Chanâs team explored de-differentiation, a process that reverts specialized, differentiated cells back to a more primitive cell. The team focused their research on multipotent stem cells, (MSCs) which can be altered into a variety of cell types through differentiation. Bone marrow MSCs have the potential to differentiate into each of the three basic types of lineage cells which form bone (osteocytes), cartilage (chondrocytes) and fat tissue (adipocytes).

The team first differentiated bone marrow MSCs towards a neuronal lineage, but then removed the differentiation conditions, allowing the cell to revert back to a form with more basic cellular characteristics.

Following this process the team recorded increased cell survival rates following transplants. In an animal model de-differentiated cells were found to be more effective in improving cognitive functions and in aiding recovery from strokes, compared to un-manipulated stem cells both in living specimens and in laboratory experiments.

The results confirm that de-differentiation is a workable technique for reengineering cells to an earlier, more primitive state but reprogrammed to have increased cell survival rates and therefore their potential for clinical use.

âœThe finding that MSCs can be reprogrammed to have enhanced survival and therapeutic efficacy in an animal model with potential application to patients is extremely exciting as it may provide a novel and clinically practical method to overcome low cell survival in cell based therapy,â concluded Dr Chan. âœWe are currently exploring other beneficial properties of the reprogrammed MSCs for other therapeutic applications.â

âœMany investigators have speculated that differentiation should improve the utility of stem cells for transplantation, but how far to differentiate the cells for the best outcome is a difficult question. Dr Chanâs team have helped provide an answer by educating mesenchymal stem cells by pre-differentiating to the desired lineage before de-differentiation, making MSCs easier to manipulate and implant,â said Dr Mark Pittenger, STEM CELLS Associate Editor.

âœInteresting questions still remain for future work such as which factors are expressed in the pre-differentiated stem cells that persist upon de-differentiation and can the de-differentiated cells be frozen for future use?â


This paper is published in STEM CELLS. Media Wishing to request a copy should contact Lifesciencenews@wiley.com or phone +44 (0)1243 770 375

Full Citation: Liu, Yang; Jiang, Xiaohua; Zhang, Xiaohu; Chen, Rui; Sun, Tingting; Fok, Kin Lam; Dong, Jianda; Tsang, Lai Ling; Yi, Shaoqiong; Ruan, Yechun; Guo, Jinghui; Yu, Mei Kuen; Tian, Yue; Chung, Yiu Wa; Yang, Mo; Xu, Wenming; Chung, Chin Man; Li, Tingyu; Chan, Hsiao, âœDedifferentiation-reprogrammed mesenchymal stem cells with improved therapeutic potentialâ, STEM CELLS, Wiley-Blackwell, October 2011, DOI: 10.1002/stem.764

Paper URL: http://doi.wiley.com/10.1002/stem.764

Contact the Author: Dr Chan can be contacted via Ms Amy Tsu, Communication Office, Chinese University of Hong Kong, amytsui@cuhk.edu.hk

About STEM CELLS: STEM CELLS, a peer reviewed journal published monthly, provides a forum for prompt publication of original investigative papers and concise reviews. The journal covers all aspects of stem cells: embryonic stem cells/induced pluripotent stem cells; tissue-specific stem cells; cancer stem cells; the stem cell niche; stem cell epigenetics, genomics and proteomics; and translational and clinical research.

For more information, please visit: http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1549-4918

STEM CELLS is co-published by AlphaMed Press and Wiley-Blackwell.

About AlphaMed Press: Established in 1983, AlphaMed Press with offices in Durham, NC, San Francisco, CA and Belfast, United Kingdom publishes two internationally renowned peer-reviewed journals: STEM CELLS®, now in its 29th year, is the world’s first journal devoted to this fast paced field of research. The Oncologist® (http://www.TheOncologist.com), also a monthly peer-reviewed publication, is in its 16th year and is devoted to community and hospital-based oncologists and physicians entrusted with cancer patient care. Both journals are premier periodicals with globally recognized editorial boards dedicated to advancing knowledge and education in their focused disciplines. In January 2012, AlphaMed Press will launch a new peer-reviewed journal, STEM CELLS Translational Medicine that will hasten the clinical applications of stem cell therapies.

About Wiley-Blackwell: Wiley-Blackwell is the international scientific, technical, medical, and scholarly publishing business of John Wiley & Sons, with strengths in every major academic and professional field and partnerships with many of the worldâs leading societies. Wiley-Blackwell publishes nearly 1,500 peer-reviewed journals and 1,500+ new books annually in print and online, as well as databases, major reference works and laboratory protocols. For more information, please visit http://www.wileyblackwell.com or our new online platform, Wiley Online Library (wileyonlinelibrary.com), one of the worldâs most extensive multidisciplinary collections of online resources, covering life, health, social and physical sciences, and humanities.


For the original version on PRWeb visit: http://www.prweb.com/releases/prweb2011/11/prweb8927493.htm

Source: prweb

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