First Candidate from Esperance’s Targeted Anti-cancer Platform, EP-100, Establishes Preclinical Proof of Concept
- Company plans initiation of clinical studies; Data to be presented at AACR -
Esperance is using its CLYP(TM) technology to develop a robust portfolio of small, novel oncolytic peptides to selectively destroy cancer cells that express target receptors. In addition to EP-100, the Company is developing several other drug candidates, all of which have a unique targeting mechanism of action whereby candidates bind specifically and exclusively to cancer cells that express the target receptors on their surfaces. The positively charged peptides disrupt the negatively charged membranes of the cancer cells, causing rapid cell death by lysis.
“These data not only support the continued development of EP-100, but also validate our core membrane-disrupting peptide platform technology,” said
“Based on the results observed in these studies, Esperance is actively planning the initiation of a Phase 1 clinical trial of EP-100 in patients with solid cancerous tumors later this year,” said
EP-100 consists of an LHRH receptor-targeting ligand conjugated to a novel membrane-disrupting peptide called CLIP 71. The drug candidate was tested in vitro and in vivo for activity in LHRH receptor over-expressing and multi-drug resistant ovarian cancer cells (OVCAR-3). As a negative control, the study utilized LHRH receptor negative SKOV-3 ovarian cancer cells. In in vitro studies designed to test the cytotoxicity of EP-100 in ovarian cancer cell lines, cells were cultured in the presence of various concentrations of EP-100 or unconjugated CLIP 71 for one to 24 hours. Results demonstrated that EP-100 was fast-acting, destroying LHRH receptor over-expressing OVCAR-3 cells within an hour compared to the unconjugated CLIP 71 which was less cytotoxic and was slow-acting (p<0.005). LHRH receptor negative SKOV-3 cells were significantly less sensitive to EP-100 than OVCAR-3 cells and the effects of EP-100 and unconjugated CLIP 71 were similar in this cell line demonstrating that EP-100 is specific for cells that over-express LHRH receptors.
In in vivo studies, the efficacy of EP-100 in comparison to saline or unconjugated CLIP 71 or cisplatinum in an ovarian cancer xenograft model (OVCAR-3) was studied. EP-100 regressed established OVCAR-3 xenografts in weekly injections at doses as low as 0.2 mg/kg bodyweight (p<0.03 vs. baseline). In comparison, tumor growth was observed across the saline control, unconjugated CLIP 71 and cisplatinum arms. In addition, in the EP-100 arm tumor volumes, weights and CA125 (a clinical biomarker for ovarian cancer) were reduced. LHRH receptor levels were also reduced and PET imaging revealed that EP-100 treated tumors became necrotic, lacking viable tumor cells after treatment. EP-100 was well tolerated in all treated groups.
About Esperance Pharmaceuticals
Esperance Pharmaceuticals, Inc. is developing a new class of targeted anticancer drugs using its Cationic Lytic Peptide (CLYP(TM)) platform technology. These drug candidates, called membrane-disrupting peptides (MDPs), selectively kill cancer cells, including cells known to be resistant to chemotherapeutic drugs, without harming normal cells. Targeting occurs through binding to specific receptors on the cell’s surface. The Company was founded on patented technology discovered by scientists at
SOURCE Esperance Pharmaceuticals, Inc.