Rare Genetic Disorder Provides Unique Insight Into Parkinson’s Disease
Pathway disrupted in Gaucher disease affects Parkinson’s-associated protein deposits, suggests novel therapeutic strategy
Massachusetts General Hospital investigators appear to have found the mechanism behind a previously reported link between the rare genetic condition Gaucher disease and the common neurodegenerative disorder Parkinson’s disease. In a report to appear in the July 8 issue of Cell and receiving early online release, they describe how disruption of the molecular pathway that causes Gaucher disease leads to the toxic neuronal deposits of the protein alpha-synuclein (Ãž±-syn) found in Parkinson’s and related disorders. In addition, rising Ãž±-syn levels further inhibit the Gaucher’s-associated pathway, leading to even more Ãž±-syn deposition, a finding that indicates therapies targeting this pathway may be a new option for patients with Parkinson’s disease.
“Our findings suggest that this molecular pathway applies not only to patients with Gaucher’s and to their relatives, some of whom may have Parkinson’s symptoms, but also to patients with the common form of Parkinson’s or other synucleinopathies,” says Dimitri Krainc, MD, PhD, of the MassGeneral Institute for Neurodegenerative Disease (MGH-MIND), the study’s senior author. “It appears that interaction between Ãž±-syn deposition and the Gaucher’s pathway forms a feedback loop that eventually leads to self-propagating disease.”
In Gaucher disease, which primarily occurs in children, patients inherit two inactive copies of the gene encoding an enzyme called glucocerebrosidase (GCase), which breaks down a lipid molecule called glucocerebroside (GlcCer). A lack of functional GCase leads to a buildup of GlcCer levels in the cellular compartments called lysosomes, in which cellular waste materials are normally digested. GlcCer accumulation in organs such as the liver, spleen, bone marrow and sometimes the brain, leads to the symptoms of Gaucher’s, which are usually treated by intravenous replacement of the missing GCase enzyme.
Clinical observations have suggested a connection between Gaucher’s and Parkinson’s disease, which affects half a million adults in the U.S. Some Gaucher’s patients or their relatives with a single defective copy of the GCase gene also develop the typical Parkinson’s symptoms of tremors, muscle rigidity and slow movement. Moreover, patients who suffer from sporadic Parkinson’s disease may also have mutations in GCase gene. In fact, mutations in that gene are currently the most common genetic risk factor for Parkinson’s disease. No previous studies, however, have directly examined how GCase mutations contribute to symptoms of Parkinson’s disease.
The MGH-MIND research team found that mutations reducing the expression of GCase lead to excess GlcCer lipid in cultured neurons. By interacting with Ãž±-syn, those GlcCer lipids induce accumulation of the protein. Brain tissue samples from a mouse model of Gaucher’s and from patients with either Gaucher’s or Parkinson’s-related disorders also revealed evidence connecting reduced GCase expression with increased Ãž±-syn deposition in neurons. In both the cultured cells and in the animal and human samples, Ãž±-syn deposits were also associated with neurodegeneration.
Since other research has indicated that overexpression of Ãž±-syn can interfere with the normal handling of proteins within cells, the MGH-MIND team also examined how Ãž±-syn might affect the movement and activity of GCase within cells. They found that accumulation of Ãž±-syn can interfere with lysosomal function by reducing the activity of GCase. This interaction between Ãž±-syn and GCase forms a feedback loop that, after a reaching a certain threshold, may lead to self-propagating disease.
“We now propose that therapies targeting glucocerebrosidase activity may help break the vicious cycle of Ãž±-syn accumulation and neurodegeneration,” says Krainc, an associate professor of Neurology at Harvard Medical School. “We think this pathway potentially applies to any disease characterized by Ãž±-syn accumulation and are now partnering with industry to develop novel therapies for Parkinson’s disease that improve targeting of glucocerebrosidase to lysosomes. We hope that such treatments may prevent or diminish the accumulation of Ãž±-syn and resultant neurodegeneration in diseases such as Parkinson’s and Dementia with Lewy bodies.”
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