October 28, 2005
study shows how good cholesterol (HDLs) provide human immunity to certain parasites
MBL, WOODS HOLE, MA -- For years biomedical researchers have known that high density lipoproteins, commonly called HDLs or "good cholesterol," are responsible for protecting humans from certain parasites, but couldn't explain how. Now MBL scientists have discovered that human HDLs work this bug-repelling magic by serving as a platform for the assembly and delivery of two naturally occurring proteins that combine to create a super-toxic antimicrobial.
The research, published in the September 30 issue of the Journal of Biological Chemistry, focuses specifically on human innate immunity to Trypanosoma brucei brucei, the parasite that gives African cattle the deadly disease called Nagana, but which doesn't harm humans even though scientists believe they are exposed to it. The parasite is a close relative of Trypanosoma brucei gambienese and Trypanosoma brucei rhodesiense, the organisms that cause African sleeping sickness in humans.
To identify the proteins--known as apolipoprotein L-1 (apo L-1) and haptoglobin-related protein (Hpr)--MBL scientists tested different amounts and combinations of the proteins on Trypanosoma brucei brucei specimens. To survive the parasite needs to digest the lipids in HDLs. Because HDL carries these proteins and enables them to combine, it is nature's perfect delivery system for the antimicrobial. And when test organisms digested the super-toxic protein mix, the single-celled organisms literally dissolved.
Shiflett and her colleagues in the MBL's Global Infectious Diseases Program are focused on understanding the molecular workings of a variety of parasites, including those that cause human African sleeping sickness, Nagana, and malaria. Such research is crucial to finding creating better treatments, and possibly cures, for diseases that are ravaging the people and economies of places like Africa and other developing countries.
Funding for this study was provided by the National Institutes of Health. The complete paper can be found in the Journal of Biological Chemistry, Volume 280, Number 38.
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