January 5, 2012
NanoCAGE Reveals Transcriptional Landscape Of The Mouse Main Olfactory Epithelium
The problem in biology of how to identify the promoters of olfactory receptor genes (>1000 genes) has remained unsolved due to the difficulty of purifying sufficient material from the olfactory epithelium. Researchers at the RIKEN Omics Science Center, collaborating with scientists from Italy, Norway, the United States, the United Kingdom and Germany, have now solved this problem using nanoCAGE technology, which enables comprehensive analysis of transcription start sites (TSSs) from tiny biological samples.
In mouse, odor is sensed by the main olfactory epithelium (MOE) by about 1100 types of olfactory receptors that are expressed by olfactory sensory neurons. Interestingly, each sensory neuron expresses only a single type of olfactory receptors, whose selective expression mechanism remains largely unknown. The population of olfactory sensory neurons that express a given olfactory receptor is small, which makes transcription analysis difficult.
Dr. Piero Carninci commented, "Combined with CAGE, nanoCAGE technology provides a new opportunity to unveil gene networks in the nervous system using omics approaches."
The research is published in the journal Genome Research.
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