Research Shows In Situ Monitoring Improves Bioprocess Control in New White Paper

March 31, 2014

A new white paper from METTLER TOLEDO explores how inline monitoring techniques and simplified spectroscopy calibration can help maximize yield, batch-to-batch reproducibility, and economics of bioproducts as demand for them continues to increase.

(PRWEB) March 31, 2014

METTLER TOLEDO is pleased to present a new installment in its white paper series on in situ spectroscopy applications. The paper, entitled “Understand and Control Bioprocesses: In Situ Analysis for Fermentation Optimization,” reviews three studies that developed simple, validated in situ spectroscopy calibration models for accurate analyte monitoring in fermentation processes – resulting in greater process control and enhanced process economics.

Inline monitoring of key process analytes such as carbon source, nitrogen source, and specific undesired metabolites can have significant impact on process understanding. The information obtained, such as optimal harvest time, can offer manufacturers significant economic rewards through enhanced productivity and quality. Additionally, inline technologies eliminate sample preparation, are non-invasive to the process, and provide real-time information on the ongoing process inside the bioreactor.

Inline monitoring has historically been seen as arduous because of the number of standards required for calibration. However, the three studies described in the paper help put this idea to rest.

Case Study 1 uses batch-fed Pencillium chrysogentum to quantify development of both Penicillin V and its precursor for enhanced yield. Case Study 2 employs a single-spectra library for simple FTIR spectroscopy calibration, gaining a 6-fold decrease in the number of standards required. Case Study 3 explores FTIR and measurement of fermentation substrate concentrations. In each case study, effective strategies were created to develop reliable, robust calibration models, enabling real-time inline monitoring of the fermentation processes.

Results included better process reproducibility, yield, and time-savings when compared to either older FTIR spectroscopy monitoring or traditional offline sampling. For information on the case studies as well as enabling ReactIR technology, download the white paper at http://www.mt.com/wp-bioprocesses.


METTLER TOLEDO provides the enabling technology, software and people that can help build a seamless workflow to translate bench scale chemistry into a commercial process. For more than 20 years, our enabling tools and services have been a strategic resource providing critical information for thousands of development scientists and engineers. Companies have used that knowledge to accelerate the discovery, development and scale-up of new chemical processes spanning the chemical and biopharmaceutical industries. For more, visit http://www.mt.com.

For the original version on PRWeb visit: http://www.prweb.com/releases/MT-BioProcess-WP/03-31-2014/prweb11715384.htm

Source: prweb

comments powered by Disqus