• E-mail
• Print
• Comment
• Font Size
• Digg
• del.icio.us
• Discuss article

New Study Examines the Potential Use of Single Molecule Detection for Nanotechnology and Quantum Information

Posted on: Tuesday, 5 February 2008, 12:00 CST

Research and Markets (http://www.researchandmarkets.com/reports/c81623) has announced the addition of Handbook of Single Molecule Fluorescence Spectroscopy to their offering.

Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analyzing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

About the Authors

Christopher Gell, School of Physics and Astronomy, University of Leeds , David Brockwell, School of Biochemistry and Microbiology, University of Leeds , and Alastair Smith, School of Physics and Astronomy, University of Leeds

ACKNOWLEDGEMENTS

GLOSSARY OF TERMS AND SYMBOLS

1 Introduction

1.1 Motivation

1.2 A historical perspective

1.3 This book

1.4 Single molecule measurements

References

2 Single molecule fluorescence techniques

2.1 Introduction

2.2 Burst analysis

2.3 Photon counting histogram

2.4 Fluorescence correlation spectroscopy

2.5 Fluorescence resonance energy transfer

2.6 Measurements of immobilized single molecules

2.7 Other related techniques

References

3 Single molecule fluorescence instrumentation

3.1 Introduction

3.2 Optical arrangements for single molecule detection

3.3 Methods for discriminating signal from noise

3.4 Wavelength or polarization selection optics

3.5 Excitation sources

3.6 Microscope objectives for single molecule fluorescence detection

3.7 Detectors for single molecule fluorescence experiments

3.8 Acquisition cards and software

3.9 Realizing single molecule instrumentation

References

4 Preparation of samples for single molecule fluorescence spectroscopy

4.1 Introduction

4.2 Dye selection

4.3 Labelling of biomolecules

4.4 Doubly labelling single protein molecules for FRET studies

4.5 Optimizing biochemical systems for single molecule

fluorescence studies

4.6 Immobilization methods

References

5 Fluorescence spectroscopy of freely diffusing single molecules: examples

5.1 Introduction

5.2 Single molecule studies of freely diffusing molecules

References

6 Fluorescence spectroscopy of immobilized single molecules: examples

6.1 Introduction

6.2 Single molecule studies of immobilized molecules

References

7 The outlook for single molecule fluorescence measurements

7.1 Outlook

References

INDEX

For more information visit http://www.researchandmarkets.com/reports/c81623

Source: Oxford University Press

Source: Business Wire

More News in this Category