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Reduction of Plastid-Localized Carbonic Anhydrase Activity Results in Reduced Arabidopsis Seedling Survivorship1[W][OA]

June 24, 2008

By Ferreira, Fernando J Guo, Cathy; Coleman, John R

Carbonic anhydrase (CA; EC 4.2.1.1) catalyzes the interconversion of CO^sub 2^ and HCO^sub 3^^sup -^ and is a major protein constituent of the C^sub 3^ higher plant chloroplast where it is presumed to play a role in photosynthetic carbon assimilation. In this study, we have used both RNA antisense and gene knockout lines to specifically reduce the activity of the chloroplast betaCA1 polypeptide (At3g01500) in the model plant Arabidopsis (Arabidopsis thaliana). Although able to germinate, seedling establishment of transgenic plants is significantly reduced relative to wild-type plants when grown at ambient levels of CO^sub 2^. Growth at elevated (1,500 [mu]L L^sup -1^) CO^sub 2^ or on plates supplemented with sucrose restores seedling establishment rates to wild-type levels. Seed from wild-type and transgenic plants exhibited no significant differences in seed protein, lipid content, or reserve mobilization during seedling growth. betaCA1-deficient seedlings do, however, exhibit reduced capacity for light-dependent ^sup 14^CO^sub 2^ assimilation prior to the development of true leaves. The small number of surviving seedlings able to grow and develop are phenotypically similar to wild-type plants, even when subsequently grown at subambient levels of CO^sub 2^. Microarray analysis of mature leaves of betaCA1-deficient plants shows some differences in transcript abundance, particularly with genes involved in ethylene signaling and response. The data suggest that reduced levels of seedling establishment by betaCA1-deficient plants could be the result of poor cotyledon photosynthetic performance at the onset of phototrophic growth and prior to the development of true leaves. Carbonic anhydrase (CA; EC 4.2.1.1) is a zinc metalloenzyme that catalyzes the reversible hydration of CO^sub 2^. With numerous isoforms, and activity found in all plants, animals, and microorganisms that have been examined, the ubiquity of its distribution implies that it plays diverse but essential roles in many biological processes (Hewett-Emmett, 2000). In terms of biochemistry, the specific requirement for CA activity is apparent. Although the uncatalyzed hydration/dehydration reactions occur, they can be sufficiently slow at physiological pH values and temperatures that the rate of interconversion limits enzymatic reactions and transport processes that require a specific inorganic species as a substrate. In addition, although equilibrium concentrations of inorganic carbon species are established by the pH, catalysis to equilibrium by CA ensures that diffusion of CO^sub 2^ across membranes, and diffusion of all inorganic carbon species within the aqueous cellular environment, is not limited by flux rates between species.

The centrality of CA in many biological processes is also demonstrated by the existence of at least five distinct CA orthologs (designated alpha, beta, gamma, delta, and epsilon) of this enzyme, potentially representing independent evolutionary origins. Although four of these proteins have type isoforms that have been associated with a specific group of organisms (alpha, vertebrates; beta, prokaryotes; gamma, archaebacteria; and epsilon, chemilithotrophs), genomic analyses have shown the presence of isoforms of more than one CA ortholog within a single organism (for example, in the cyanobacteria [Soltes-Rak et al., 1997] as well as higher plant species). In the C^sub 3^ plant Arabidopsis (Arabidopsis thaliana), partial genome analysis identified at least 14 genes likely to encode CA isoforms representing the alpha, beta, and gamma families (Moroney et al., 2001). Most recently, EST and gene expression analyses based on the full genome have shown that three of eight alpha-type sequences are expressed in Arabidopsis, whereas all six of the beta-type sequences are represented in the EST database (Fabre et al., 2007). In addition, Parisi et al. (2004) identified a family of three gamma-type sequences in Arabidopsis that are all expressed.

In higher plants, little is known about expression and function of alphaCAs. Reverse transcription-PCR analysis using Arabidopsis RNA identified broad tissuespecific patterns of expression for alphaCA1 (At3g52720), alphaCA2 (At2g28210), and alphaCA3 (At5g04180; Fabre et al., 2007), and alphaCA1 has been shown to be localized to the chloroplast stroma following transport through the secretory pathway and N-glycosylation (Villarejo et al., 2005). No specific roles for these proteins have been described. In Arabidopsis, members of the gammaCA family are localized to the mitochondria and appear to play a role in respiration. Disruption of At1g47250, one of three nucleus-encoded gammaCA isoforms, results in a reduction in the abundance of mitochondrial complex I and supercomplex I and III^sub 2^ and a concomitant reduction in respiratory activity (Perales et al., 2005). Most plant research, however, has focused on the characterization and assignment of function for the more abundant beta-family isoforms. In C^sub 4^ plants, a mesophyll cell- localized betaCA is required for the hydration of CO^sub 2^ to provide HCO^sub 3^^sup -^ for phosphoenolpyruvate carboxylase (PEPCase), the initial carboxylation reaction of this CO^sub 2^- concentrating pathway (Von Caemmerer et al., 2004). Transgenic Flaveria with mesophyll cell CA activities below 10% of wild-type levels had low rates of CO^sub 2^ assimilation and grew poorly at ambient levels of CO^sub 2^. In an analogous but nonphotosynthetic role, betaCA isoforms have been localized to nodules of legumes where they are presumed to catalyze the synthesis of HCO^sub 3^^sup – ^ for use by PEPCase in its anaplerotic role as the source of C-4 acids for amination reactions or bacteroid carbon catabolism (Flemetakis et al., 2003). In addition, the synthesis of nodule localized C-4 acids (with the coordinated expression of CA and PEPCase) has also been postulated as part of the mechanism to control gas diffusion within this tissue (Atkins et al., 2001). Other nonphotosynthetic roles for the higher plant enzyme include a requirement for a plastid localized betaCA isoformidentified in dark- grown cotton (Gossypium hirsutum) seedlings that appears to provide HC^sub 3^^sup -^ for CoA carboxylase in lipogenesis (Hoang et al., 1999). Expression of this CA was correlated with the period of storage lipid accumulation in maturing embryos, and inhibition of CA activity reduced incorporation of radiolabeled acetate into lipids (Hoang and Chapman, 2002).

The majority of CA activity in C^sub 3^ higher plants, however, is found in photosynthetic tissue, primarily leaves. In earlier studies, two isoforms of a beta-type CA were identified: a highly abundant chloroplast-localized enzyme and a less well-expressed cytosolic form (Kachru and Anderson, 1974; Fett and Coleman, 1994; Rumeau et al., 1996). In Arabidopsis, both forms are nucleus encoded and the chloroplast-localized enzyme (betaCA1) contains a transit peptide, which is removed following entry into the plastid, whereas the cytosolic isoform (betaCA2) is unprocessed following translation (Fett and Coleman, 1994). Like other chloroplast-localized proteins involved in photosynthesis, the expression of betaCA1 is light regulated and/or chloroplast development dependent, whereas the expression of betaCA2 is not (Fett and Coleman, 1994). A recent highly detailed study of patterns of expression of the six betaCA isoforms in Arabidopsis confirmed the tissue and cellular localization of betaCA1 and betaCA2, but also showed that expression of other betaCA isoforms occurred in above-ground tissue (Fabre et al., 2007). A role for the cytoplasmic localized CA activity in the leaf has not been identified; however, betaCA2 (and other cytoplasmic isoforms) may be required for synthesis of HCO^sub 3^^sup -^ for use by anaplerotic enzymes, such as PEPCase, to speed the solubilization of gaseous CO^sub 2^ from leaf air spaces and to assist in the diffusion of CO^sub 2^/HCO^sub 3^^sup -^ across the cytosol to the chloroplast. Proposed photosynthetic roles for the highly abundant stromal enzyme betaCA1 have included facilitation of CO^sub 2^ movement across the chloroplast envelope and maintenance of maximal rates of CO^sub 2^ and HCO^sub 3^^sup -^ diffusion through the stroma by rapidly equilibrating C^sub i^ speciation (Badger and Price, 1994). Additional roles may include provision of CO^sub 2^ to Rubisco by catalyzing the dehydration of HCO^sub 3^^sup -^ in the alkaline stroma in close proximity to the principal CO^sub 2^-fixing enzyme. In support of this role, it has been shown that at least a portion of a chloroplast betaCA1 protein and activity is associated with the Rubisco-containing Calvin cycle enzyme complex (Jebanathirajah and Coleman, 1998). An additional proposed role for chloroplastic CA activity includes modulation of the stromal pH in which catalyzed CO^sub 2^/HCO^sub 3^^sup -^ interconversion could protect against lightinduced pH transients.

In addition to the stromal-localized betaCA, several studies also suggest that another CA activity is associated with the thylakoids where it is presumed to provide bicarbonate for PSII activity (Stemler, 1997). In both C^sub 3^ and C^sub 4^ plants, membrane- bound CA activity, distinct from the stromal betaCA1 isoform, can be measured in PSII complex preparations (Lu and Stemler, 2002; Pronina et al., 2002). Data have also been presented that describe CA activity associated with the PSII OEC33 polypeptide from pea (Pisum sativum); however, this protein exhibits no sequence similarity to any known CA family and a specific role for this activity in PSII has not yet been identified (Lu et al., 2005). Recently, an Arabidopsis betaCA5-GFP fusion protein was shown to be targeted to chloroplasts, although a specific association with thylakoids was not identified (Fabre et al., 2007). Attempts to show that the chloroplastic CA activity in C^sub 3^ higher plant leaves plays a role in photosynthesis have included both antisense RNA and chemical inhibitor studies in which the effect of reduced leaf CA activity on photosynthesis was examined. Oxygen electrode studies following treatment of leaf segments with the CA inhibitor ethoxyzolamide resulted in a 30% to 50% decline in rates of O^sub 2^ evolution at external CO^sub 2^ concentrations approaching the compensation point (Badger and Pfanz, 1995). Increasing external CO^sub 2^ concentrations reduced the level of inhibition. In contrast, antisense RNA studies in which the foliar levels of CA have been reduced to 1% to 2% of wild-type activity generated mature tobacco (Nicotiana tabacum) plants that displayed almost no discernable phenotype and only a small reduction in photosynthesis (Majeau et al., 1994; Price et al., 1994). Gas exchange measurements indicated that chloroplast CO^sub 2^ concentrations were reduced by approximately 20 [mu]mol mol^sub -1^, a decline that would not have a profound impact on the rate of CO^sub 2^ fixation (Price et al., 1994; Williams et al., 1996). In an attempt to further examine the role of higher plant CAs in photosynthesis and other metabolic processes, we have extended specific CA activity modification studies to Arabidopsis, the model plant system from which we have previously characterized the dominant stromal chloroplastic and cytosolic CA isoforms (Fett and Coleman, 1994).

RESULTS

Antisense RNA targeted against betaCA1 (At3g01500) and isolation of a homozygous betaca1 knockout line (Salk_0106570) were both used to specifically reduce the activity of the major foliar, chloroplast- localized isoform of CA in C^sub 3^ plants. The gene organization, location of PCR primers used for characterization, and position of the T-DNA insertion in the Arabidopsis betaCA1 gene are shown in Figure 1A. Activity measurements identified a number of antisense lines with reduced CA levels, and a single line showing the lowest expression of the betaCA1 protein was selected for further study. Western-blot analysis of soluble protein isolated from actively growing, intact 10-d-old seedlings grown on one-half-strength Murashige and Skoog (MS) plates, as well as CA activity measurements, showed that both antisense (betaca1-AS) and knockout (betaca1) lines had significantly altered the expression of betaCA1 (Fig. 1, B and C) with similar reductions of betaCA1 expression and total CA activity observed in even younger plants, such as 4- and 6- d-old seedlings. As shown in Figure 1, the abundance of 25-kD betaCA1 protein is significantly reduced in the antisense line and absent in the knockout line. In contrast, the abundance of the cytosolic 28-kD betaCA2 polypeptide (At5g14740; Fett and Coleman, 1994) when compared with wild-type levels is unaffected in these two lines. In concert with the western analysis, measurement of seedling total CA activity showed that residual CA activity in betaca1-AS was approximately 30% of wild-type levels, whereas betaca1 was

As the onset of seedling stagnation usually occurred within 4 to 6 d postgermination, the availability and mobilization of seed reserves and/or early photosynthetic capacity may be determining survivorship. SDSPAGE analysis of dry seed and seedling soluble proteins over the first 5 d following imbibition did not reveal any obvious qualitative or quantitative differences in protein profiles between the betaca1 knockout line and wild-type plants (Fig. 3A). Analysis of dry seed total lipid content isolated from equal numbers of seeds did not reveal any significant differences in quality or quantity between the betaca1 and wild-type lines (data not shown). In addition, storage lipid catabolism was seemingly unaffected by the reduced CA activity. Levels of eicosenoic acid (20:1), a fatty acid specific to storage triacylglycerol (Lemieux et al., 1990), exhibited similar rates of decline when measured in betaca1 and wild- type germinating seed and seedlings (Fig. 3B). Germination and growth of betaca1 knockout and antisense plants in the dark, in terms of hypocotyl and root length, were also not significantly different from dark-grown wild-type plants, suggesting that mobilization of reserves is unaffected by lower CA activity (data not shown). Transfer of these plants, however, following 4 d of dark growth to the light for 14 d resulted in significant mortality of the CA activity-reduced seedlings when grown on minimal medium (Fig. 3C). The provision of 1% Suc in the plates eliminated seedling mortality under these conditions.

As these data suggested that the mutant phenotype is light dependent, it is possible that the photosynthetic capacity of the seedlings has been affected by the reduction of CA activity. Although photosynthetic gas exchange analysis of very young Arabidopsis seedlings is not easily achieved, the relative capacity for light-dependent CO^sub 2^ assimilation, as measured by uptake of ^sup 14^CO^sub 2^ into acid-stable products at ambient levels of total CO^sub 2^, was assessed (Table I). Although exhibiting similar rates of ^sup 14^CO^sub 2^ assimilation 2 d postimbibition, 3- and 4- d-old betaca1 antisense and knockout seedlings had significantly reduced capacity for ^sup 14^CO^sub 2^ incorporation when compared with wild-type plants. Rates of ^sup 14^CO^sub 2^ fixation in the dark were very low (

It is apparent, however, that not all seedlings with reduced or absent betaCA1 activity require Suc or high CO^sub 2^ for growth and development. As can be seen in Figure 2A, some of the plants are able to continue growing and develop true leaves and will eventually flower and set seed when grown at air levels of CO^sub 2^. As shown in Figure 4, surviving betaca1 knockout seedlings grown to maturity at ambient levels of CO^sub 2^ expressed no betaCA1 protein and had low levels of foliar CA activity. Residual activity is approximately 28% of wild-type levels and presumably represents the activity primarily associated with betaCA2 as well as other active isoforms. Phenotypically, the betaca1 plants cannot be distinguished from wild- type plants. Growth at less than ambient levels of CO^sub 2^ (150 [mu]L L^sup -1^) does result in a decrease in plant size and developmental delay of flowering in wild-type and betaca1 plants; however, no obvious phenotypic differences were noted between betaca1 and wild-type lines.

Given the morphological phenotypic similarity of mature betaca1 knockout and wild-type plants, transcript profiling was undertaken to determine if there were any identifiable differences in transcript levels between the two genotypes. It was possible that compensatory changes in gene expression, including other CA isoforms, could have resulted in plants that are phenotypically similar to wild type. As shown in Table II, microarray analysis using mature leaf tissue from air-grown plants showed that expression of other CA isoforms in mature leaves was not modified in response to the specific reduction of betaCA1. Assuming comparable levels of hybridization efficiency between the Affymetrix ATH1 chip gene-specific probe sets, the microarray data also show that transcripts for both betaCA1 and betaCA2 proportionally constitute the majority of CA-specific mRNAs in wild-type leaves when compared with other CA isoforms. In contrast to the other CA-encoding genes, microarray analysis revealed that 128 genes displayed significant changes in transcript levels, with 125 genes expressed in betaca1 plants at 0.55-fold or less than wild-type plants and three genes expressed at levels of 1.8-fold or higher than those found in wild- type plants (Supplemental Table S1). Given the presumptive role(s) of betaCA1 in chloroplast metabolism, it was noted that there were no significant differences between wild-type and betaca1 plants in expression of genes known to encode proteins with primary roles in photosynthesis, Calvin cycle activity, or Suc/starch biosynthesis and catabolism. There did seem to be an overrepresentation of significantly down-regulated genes that have been identified as members of the ETHYLENE RESPONSE FACTOR (ERF) gene family and/or responsive to ethylene. Using a recently described phylogenetic system for grouping (Nakano et al., 2006), the down-regulated ERF genes were identified as AtERF#058 (At1g22190) in Group I; AtERF#012 (At1g21910) and AtERF#018 (At1g74930) in Group II; AtERF#078 (At3g15210) in Group VIII; and AtERF#100 (At4g17500), AtERF#101 (At5g47220), AtERF#103 (At4g17490), AtERF#104 (At5g61600), AtERF#105 (At5g51190), and AtERF#107 (At5g61590) in Group IX. In addition to these ERF genes, two ethyleneresponsive MYB transcription factors, AtMYB73 (At4g37260) and AtMYB77 (At3g50060), as well as a gene encoding a 1-aminocyclopropane-1-carboxylic acid (ACC) synthase isoform, ACS6 (At4g11280), were also significantly down-regulated. At5g45340, which encodes an enzyme implicated in abscisic acid catabolism (abscisic acid 8#-hydroxylase, CYP707A3), was also significantly down-regulated in the betaca1 plants (Umezawa et al., 2006). It was shown recently that expression of a rice (Oryza sativa) homolog of the Arabidopsis gene is regulated by ethylene (Saika et al., 2007). Three genes showed significant increases in transcript abundance in the betaca1 plants (Supplemental Table S1). These were:At1g69530, encoding an expansinlike protein; the plastid gene AtCg00420, encoding ndhJ; and At4g38840, an auxin-responsive gene. The process by which reduced betaCA1 activity increases transcript abundance of these three genes is unclear. In addition, no obvious plant phenotypes were observed to be associated with these transcript changes.

DISCUSSION

Both antisense and gene knockout strategies have been used in this study to significantly reduce or eliminate specifically the chloroplast-localized activity of the betaCA1 protein in Arabidopsis. The CA activity remaining in these plants would appear to be primarily associated with the cytosolic isoform betaCA2, which was unaffected in these transgenic lines, although other betaCA isoforms (or other CAs from the other identified gene families, alpha and gamma) would also contribute to the total remaining activity in the leaf. Transcript profiling shows that five identified betaCA as well as three gammaCA and at least one alphaCA isoforms are expressed in the leaf, in agreement with a recent study where reverse transcription-PCR also identified betaCA3 and two additional expressed alphaCAs as leaf transcripts (Fabre et al., 2007); however, betaCA1 and betaCA2 represent approximately 75% of the total CA-specific transcripts identified by microarray analysis.

From the data presented in Figure 2, it is apparent that survivorship of the betaca1-AS and betaca1 Arabidopsis seedlings is significantly lower than wild-type plants. That elevated CO^sub 2^ is able to rescue these seedlings indicates that the reduced CA activity is likely responsible for the inadequate provision of either CO^sub 2^ or HCO^sub 3^^sup -^ for an important metabolic process(es). The ability of Suc to also restore survivorship to wild- type levels could suggest that it is availability or mobilization of carbon reserves in the seedlings that is limiting growth and survivorship. Many Arabidopsis mutants with reduced capacity to mobilize lipid reserves exhibit a Suc-dependent phenotype for postgermination growth and development, particularly in the dark (Penfield et al., 2005). Analysis of both dry seed protein and total lipid content of the transgenic lines did not reveal any significant differences, in terms of both quantity and quality, when compared with wild-type seed, suggesting that the availability of seed reserves was not limiting early growth (Fig. 3). Polypeptide profiles of young seedlings, prior to the arrest of development seen in the transgenic lines, were also not significantly different. Levels of eicosenoic acid (20:1), specific to storage triacylglycerol, decreased in the betaCA1-reduced lines at the same rate as in wildtype seedlings, suggesting that storage lipid catabolism was unaffected. In addition, dark growth, in terms of extent of hypocotyl elongation, was unaffected in the transgenic lines, whereas the seedling establishment phenotype became readily apparent when these lines were transferred into a light environment in the absence of Suc (Fig. 3). The mobilization of reserves in the early stages of growth could have required CA activity; however, the inhibitory impact of high light levels on seedling establishment in the betaCA1-reduced plants and the ability of high CO^sub 2^ concentrations to rescue seedling development suggest a photosynthetic rather than metabolic role for the betaCA1 isoform at this developmental stage. It is possible that low chloroplastic CA activity restricts CO^sub 2^ gas exchange in the thicker tissue of the cotyledons (as compared with true leaves). Arabidopsis cotyledons are known to have lower and more variable stomatal densities relative to true leaves (Geisler and Sack, 2002; Teng et al., 2006) and thus may have increased diffusive resistance for CO^sub 2^. Reduced intracellular CO^sub 2^ could limit Calvin cycle activity and result in reduced levels of photosynthate for growth, increased levels of photoinhibition (particularly at high light levels), and, ultimately, potential stagnation of seedling growth and development. The measurements of photosynthetic assimilation obtained using ^sup 14^CO^sub 2^ support the hypothesis of reduced cotyledon carbon fixation in the betaCA1-reduced plants, particularly at a point in development where embryo reserves are limited and phototrophy is contributing a larger proportion of carbohydrates for growth. It was anticipated that growth at less than ambient CO^sub 2^ (150 [mu]L L^sup -1^) would reduce survivorship to even lower levels that observed at ambient CO^sub 2^ concentrations; however, although trending lower, the differences were not statistically significant. It is possible that levels of CO^sub 2^ in the closed plates were somewhat higher than the subambient chamber CO^sub 2^ levels, or that a much greater reduction in chamber CO^sub 2^ level would be required to impact on survivorship beyond that observed at ambient levels. Ultimately, individual seed variation in available reserves, seedling variation in diffusive resistance, or rate of development may allow some individuals to escape this early stage of growth restriction and subsequently develop leaves. The carbon assimilation capacity of those transgenic plants that survive to produce true leaves does not seem to be compromised by low chloroplastic CA activity, as evidenced by their wild-type growth rate and phenotype at ambient and even subambient CO^sub 2^ levels (Fig. 4). The plant phenotypes were comparable over a range of photoperiods, including 16- and 12- h days (data not shown), even as short as 8 h (Fig. 4A). The absence of a phenotype in reduced CA activity plants that are able to establish themselves and continue to grow is similar to what was seen in previous studies. Mature betaCA1 antisense tobacco plants in which foliar CA activities were reduced to less than 2% of wild- type activities were reported to be phenotypically similar towild- type plants with respect to growth and development (Majeau et al., 1994; Price et al., 1994). Short-term, on-line gas exchange analysis showed that reduced chloroplastic CA activity resulted in less discrimination against ^sup 13^CO^sub 2^ than that obtained for wild- type plants. These data were consistent with a reduction in CA antisense plant chloroplastic CO^sub 2^ levels that would have resulted in only a small decline (

Microarray analysis did not reveal any compensatory changes in the expression of other CA isoforms or any significant changes in expression patterns of genes involved in photosynthesis and carbon metabolism in mature leaves of the betaca1 plants. These data (in addition to the mutant phenotype) suggest that CO^sub 2^/HCO^sub 3^^sup -^ exchange is not limiting in the absence of betaCA1 activity in the chloroplast or that existing carbon metabolic capacity is sufficiently plastic to not require a transcriptional response. Recently, the CA isoform betaCA5 has been reported to be also localized to the chloroplast and may provide sufficient CA activity for adequate catalysis of plastid CO^sub 2^/HCO^sub 3^^sup – ^ exchange in mature leaves (Fabre et al., 2007). Transcript levels for this protein, however, are very low relative to betaCA1, and the abundance or activity of this specific isoform is unknown. Microarray analysis indicted that there were no other readily discernible patterns in transcript abundance differences between wild-type and betaca1 plants other than the unusual overrepresentation of downregulated ERF transcripts and other ethylene-response and -regulated genes in betaca1 leaves. The reasons for this are unclear. Up-regulation of the ERF transcription factors is usually in response to ethylene exposure and/or biotic stresses such as wounding or response to pathogens and results in the regulation of expression of subsets of genes containing GCC boxes within their promoter regions (Fujimoto et al., 2000; Onate- Sanchez and Singh, 2002; McGrath et al., 2005; Yang et al., 2005). Similarly, both MYB73 and MYB77 exhibit increased transcript abundance in response to ethylene as well as other stimuli, including jasmonic acid and salicylic acid (Yanhui et al., 2006). The observation of expression levels of these ERF and MYB genes well below wild-type levels in the betaca1 plants could be the result of an attenuation of the basal levels of signaling. It is interesting to note that the final enzymatic step in the ethylene biosynthetic pathway, catalyzed by ACC oxidase, occurs when ACC is oxidized to ethylene, HCN, and CO^sub 2^. In vivo and in vitro ethylene biosynthesis is dependent on the presence of CO^sub 2^ (Kende, 1993), and enzyme mechanism studies have shown a requirement for CO^sub 2^/HCO^sub 3^^sup -^ in the generation of the productive oxidant in ACC oxidase catalysis (Rocklin et al., 2004). It is possible that reduced intracellular CA activity reduces the availability of the appropriate inorganic species for optimal ACC oxidase catalysis with the resultant lower levels of ethylene and the observed transcriptome response. Alternatively, CA activity may be required for some other aspect of ethylene/jasmonic acid signaling that involves the identified subset of ERF, MYB, and ACS genes. A study of the impact of reduced CA activity on ethylene biosynthesis and other aspects of ethylene/jasmonic acid signaling pathways is under way.

In conclusion, we have demonstrated that betaCA1 plays a significant role in seedling establishment in Arabidopsis and that its impact is likely promulgated by maintaining the photosynthetic capacity of the cotyledons through the initial stages of seedling photoautotrophic growth and prior to the development of the first true leaves.

MATERIALS AND METHODS

Plant Material and Growth Conditions

Following stratification and imbibition of sterilized seeds for 4 d at 4[degrees]C, Arabidopsis (Arabidopsis thaliana) ecotype Columbia was grown in sterilized artificial soil (Pro-Mix BX) saturated with 1 g L^sup -1^ of 20:20:20 all-purpose plant fertilizer (PlantProd) or on 0.8% agar, one-half-strength MS plates (supplemented with 1% [w/v] Suc when required) at 22[degrees]C and a light intensity of 120 [mu]mol m^sup -2^ s^sup -1^ unless otherwise indicated, and as described previously (Fett and Coleman, 1994). Chamber CO^sub 2^ concentrations were determined by infrared gas analyzers (Horiba), which controlled rates of either CO^sub 2^ supplementation or the activity of a soda-lime scrubbing system to achieve the desired CO^sub 2^ level. At steady state, CO^sub 2^ concentrations could be maintained in the chambers at the desired set points of 150 and 1,500 [mu]L L^sup -1^ +- 30 [mu]L L^sup -1^. Ambient (nonmodified) CO^sub 2^ levels were routinely measured at 410 [mu]L L^sup -1^ +- 15 [mu]L L^sup -1^.

Molecular Biology

The betaCA1 antisense vector was generated by cloning a 1.2-kb EcoRI cDNA fragment (Fett and Coleman, 1994) containing the intact betaCA1 (At3g01500) coding region into the binary vector pGA643 (An et al., 1989). Restriction digest and PCR analysis was used to confirm the antisense orientation of betaCA1 in pGA643. Agrobacterium tumefaciens strain LBA4404 was transformed by electroporation (Gene-Pulser; Bio-Rad) and transformants selected for growth on Luria-Bertani media containing tetracycline (12 [mu]g mL^sup -1^) and streptomycin (30 [mu]g mL^sup -1^). Four-week-old, soil-grown Arabidopsis was transformed by Agrobacterium containing the appropriate constructs using vacuum infiltration (Bechtold et al., 1993), and transgenic seed (T1) was identified by growth on one- half-strengthMS plates containing 1%Suc and 25 [mu]g mL^sup -1^ kanamycin. T4 seed was produced by three rounds of selfing with the initial 2 weeks of growth of each generation on one-half-strength MS plates containing kanamycin and Suc followed by transfer to pots for seed production. Plants from T3 seed lines were tested for reduced foliar CA activity and the T4 seed generated by individual lines with the lowest measured activity used for all studies.

Seeds for the betaCA1 (At3g01500) knockout line were obtained from the Arabidopsis Biological Resource Center (The Ohio State University, Columbus, OH) as a T-DNA insertion line (Salk _0106570) and grown on one-half-strength MS plus Suc plates as described above. PCR analysis using genomic DNA and forward primer 5′- TGCCTTCGTGGTCCGTAACAT-3′ and 5′-TCAAACCATAAATACAACCGATTTG-3′ (specific for At3g01500) and primer LBaI 5′-TGGTTCACGTAGTGGGCCATCG- 3′ (specific for T-DNA left border) followed by sequence analysis of the products was used to identify those plants carrying the insertion at At3g01500. The PCR program used for amplification was as follows: one cycle of predenaturation at 94[degrees]C for 3 min, followed by 35 cycles at 94[degrees]C for 30 s, 58[degrees]C for 40 s, 72[degrees]C for 1 min, and a final extension at 72[degrees]C for 10 min. Homozygous lines containing a single T-DNA insert in At3g01500 were obtained by back-crossing with wild-type plants followed by two rounds of self-fertilization and progeny analysis on one-halfstrength MS plates containing 25 [mu]g mL^sup -1^ kanamycin and 1% Suc.

RNA Sampling and Microarray Analysis

Discs from the area flanking the midvein of fully expanded leaves (leaf 6) of 20-d-old plants grown at 22[degrees]C, air levels of CO^sub 2^, a light intensity of 120 [mu]mol m^sup -2^ s^sup -1^, and a photoperiod of 16 h light/8 h dark were harvested from nine individual plants of each genotype with samples from three individuals pooled as biological replicates. Following harvesting, samples were immediately frozen in liquid N^sub 2^ and RNA extracted using an RNAeasy Plant Mini kit (Qiagen) according to the manufacturer’s instructions. cDNA synthesis, labeling, and hybridization to GeneChip Arabidopsis ATH1 genome arrays was carried out using standard Affymetrix protocols (http://Affymetrix.com) at the Botany Affymetrix GeneChip Facility, University of Toronto. Following scanning (Affymetrix GCS 3000) of the six chips (three biological replicates per genotype), the raw data were normalized using the GeneChip Operating System software with the scaling factor set at 500. Hybridization signals for all genes on each chip were examined, and where the signal level was below background level (declared absent by Affymetrix Microarray Suite), that gene was eliminated from further analysis. Reproducibility between replicate samples was tested by comparing hybridization signals for each gene not declared absent and calculating the least square regression. All replicates for each genotype showed acceptable reproducibility (R^sup 2^ > 0.9). All genes (except those declared absent) were used in the Significant Analysis of Microarrays program (Tusher et al., 2001), where the two class unpaired response option was used to compare hybridization signals of the two genotypes (wild type versus betaca1) with the measure of significant fold change set at 1.8 and a false discovery rate

Protein Biology and Biochemistry

Chlorophyll assays, CA assays (Wilbur and Anderson, 1948), and western analysis of soluble proteins obtained from intact seedlings or mature leaf discs were as described previously (Majeau and Coleman, 1994). For seed protein and lipid analysis, 50 seeds of each Arabidopsis line to be tested were extracted in equal volumes of the appropriate buffer to allow for quantitative as well as qualitative comparisons. Extraction and SDS-PAGE analysis of seed proteins was as described by Keith et al. (1994). Techniques used for the extraction, methanolysis, and gas chromatographic identification and quantification of lipids in each 50-seed aliquot were as described by Khan and Williams (1993) and Zhang et al. (2001).

^sup 14^CO^sub 2^ Incorporation by Seedlings

Two-, 3-, and 4-d-old seedlings (postimbibition/stratification) growing on one-half-strength MS plates were exposed to ^sup 14^CO^sub 2^ (obtained by acidification of 200 [mu]Ci of NaH^sup 14^CO^sub 3^ stock [SA 56 mCi mmol^sup -1^]; ICN) for 15min at ambient levels of CO^sub 2^ and O^sub 2^ in a sealed glass vessel at 22[degrees]C and a light intensity of 350 [mu]mol m^sup -2^ s^sup – 1^. Eight intact seedlings per sample, with four replicates per line at each time point, were frozen in liquid N^sub 2^, ground to a fine powder, and suspended in 200 [mu]L of acidified water (1 mM acetic acid). Aliquots were added to 5 mL of ACS scintillation mixture (Amersham) and the disintegrations per minute incorporated determined using a Beckman LS 6000IC counter following correction for efficiency of sample counting.

Supplemental Data

The following materials are available in the online version of this article.

Supplemental Figure S1. Recovery of air-grown betaca1 seedlings following transfer to high CO^sub 2^.

Supplemental Table S1. Microarray analysis of RNA isolated from leaves of betaca1 plants.

Received March 5, 2008; accepted April 4, 2008; published April 23, 2008. 1 This work was supported by the Natural Sciences and Engineering Research Council of Canada (to J.R.C.).

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Fernando J. Ferreira2,3, Cathy Guo2, and John R. Coleman*

Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3B2

2 These authors contributed equally to the article.

3 Present address: Department of Biology, University of North Carolina, Chapel Hill, NC 27599.

* Corresponding author; e-mail coleman@csb.utoronto.ca.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: John R. Coleman (coleman@csb.utoronto.ca).

Copyright American Society of Plant Biologists Jun 2008

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