January 24, 2011
EMBL Software Runs Experiments On Its Own
The sight of a researcher sitting at a microscope for hours, painstakingly searching for the right cells, may soon be a thing of the past, thanks to new software created by scientists at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany. Presented today in Nature Methods, the novel computer programme can rapidly learn what the scientist is looking for and then takes over this laborious and time-consuming task, automatically performing complex microscopy experiments when it detects cells with interesting features.
Called Micropilot, the software brings machine learning to microscopy. It analyses low-resolution images taken by a microscope and, once it has identified a cell or structure the scientists are interested in, it automatically instructs the microscope to start the experiment. This can be as simple as recording high-resolution time-lapse videos or as complex as using lasers to interfere with fluorescently tagged proteins and recording the results.
Jan Ellenberg and Rainer Pepperkok, whose teams at EMBL designed Micropilot, have used the software to deploy several different microscopy experiments, investigating various aspects of cell division. They determined when structures known as endoplasmic reticulum exit sites form, and uncovered the roles of two proteins, CBX1 and CENP-E, in condensing genetic material into tightly-wound chromosomes and in forming the spindle which helps align those chromosomes. This software will be a key tool for the European systems biology projects Mitosys and SystemsMicroscopy, for which Ellenberg and Pepperkok are developing technology.
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