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Influence Of Pulp Extraction Technique On DPSCs Quality And Quantity

March 21, 2011

Today, during the 89th General Session & Exhibition of the International Association for Dental Research, held in conjunction with the 40th Annual Meeting of the American Association for Dental Research and the 35th Annual Meeting of the Canadian Association for Dental Research, lead researcher C. Paganellii will present a poster titled “Influence of Pulp Extraction Technique on DPSCs Quality and Quantity.”

The objective of this study was to compare the quality and quantity of dental pulp stem cells (DPSC) obtained from permanent teeth using two different pulp extraction techniques. In this study, 28 upper premolars, with no caries or periodontal problems and extracted for orthodontic reasons, were randomly divided in two different groups. Teeth of group A were longitudinally cut in two half parts with a rotating diamond disc under cool water irrigation whereas in the group B, after the creation of a 3mm deep and 40° angled notch covering all the tooth perimeter obtained thanks to the use of a dedicated bur, an Instron Testing Machine was used in order to create a fragile fracture after the application of a controlled load.

During the study, pulp tissue was removed, digested in collagenase and dispase, filtered and cells were cultured in alpha-modified minimum essential culture medium, supplemented with 20% FCS, 100µM 2P-ascorbic acid and 2 mM L-glutamine. The researchers were able to extract and cultivate DPSC’s from every dental element. The stem cell integrity, growth rate, quality and quantity were higher in group B samples.

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