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Cancer Therapy; Human papillomavirus L1 renders cells susceptible to NK-mediated destruction

Posted on: Thursday, 12 February 2004, 06:00 CST

2004 FEB 20 - (NewsRx.com & NewsRx.net) -- Human papillomavirus L1 renders cells susceptible to NK-mediated destruction.

According to new research from Poland, "the aim of the present study was to characterize natural cell-mediated cytotoxicity against COS-7 cells transfected with potentially oncogenic HPV-8 L1 DNA sequences cloned in sense and antisense orientation and to evaluate their lysis by peripheral blood lymphocytes (PBL) from patients with epidermodysplasia verruciformis (EV), a rare disease associated with life-long infection by specific HPV types."

"COS-7 cells were transfected with HPV-8 Hinc II restriction fragment (nucleotide positions 5434-7654) cloned in sense (COS-LIS) and antisense (COS-LIA) orientation into pCB6 expression vector. Cytotoxic activity of isolated PBL against COS cell lines as well as K562 erythroleukaemic cells was evaluated by Cr-51-release assay," described M. Oldak and colleagues, Medical University of Warsaw, Center for Biostructural Research.

"We found that lymphocytes responsible for natural lysis of COS and K562 cells are CD3-negative CD56-positive natural killer (NK) cells. Analysis of NK cell cytotoxic activity against different COS cell lines has revealed that lymphocytes from healthy subjects killed COS-LIS cells significantly more efficiently than wild COS-7 and COS-LIA cells. Significantly more efficient lysis of COS-LIS cells was also observed in EV patients."

"Thus, expression of HPV L1 renders target cells more susceptible to NK-mediated cytotoxicity that may enable more effective elimination of transformed cells," researchers concluded.

Oldak and colleagues published the results of their research in International Journal of Molecular Medicine (Natural cell-mediated cytotoxicity of peripheral blood lymphocytes against target cells transfected with epidermodysplasia verruciformis-specific human papillomavirus type 8 L1 DNA sequences. Int J Mol Med, 2004;13(1):187-191).

For additional information, contact M. Oldak, Med University Warsaw, Center Biostruct Research, Department Histol & Embryology, Molecular Cell Biology Laboratory, Chalubinskiego 5, PL-02004 Warsaw, Poland.

The publisher of the International Journal of Molecular Medicine can be contacted at: Professor D.A. Spandidos, 1, S. Merkouri St., Editorial Office, Athens 116 35, Greece.

The information in this article comes under the major subject areas of Biotechnology, DNA Research, Gene Therapy, Oncology, and Virology.

This article was prepared by Drug Week editors from staff and other reports. Copyright 2004, Drug Week via NewsRx.com & NewsRx.net.

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