Diagnostics; Method for thermodynamic analysis of protein-DNA interactions developed
Posted on: Thursday, 26 February 2004, 06:00 CST
2004 MAR 5 - (NewsRx.com & NewsRx.net) -- Scientists have developed a new H/D exchange- and mass spectrometry-based method for thermodynamic analysis of protein-DNA interactions.
According to recent research published in the journal Chemistry and Biology, "The application of SUPREX (stability of unpurified proteins from rates of H/D exchange) to the thermodynamic analysis of protein-DNA complexes is described. A series of five model protein-DNA complexes involving two known DNA binding proteins, Arc repressor and CopG, were analyzed in order to determine the accuracy, precision, and generality of the SUPREX technique for quantifying the strength of protein-DNA interactions."
"For protein-DNA complexes that reversibly unfold in a two-state manner, we demonstrate that reasonably precise K[sub]d values in agreement with those determined by conventional techniques can be determined by SUPREX," said Liyuan Ma and Michael C. Fitzgerald at Duke University in the United States. "In the case of protein-DNA complexes that are not well modeled by a two-state unfolding mechanism, we find that relative binding affinities can be determined in the SUPREX experiment."
Ma and Fitzgerald published their study in Chemistry and Biology (A new H/D exchange- and mass spectrometry-based method for thermodynamic analysis of protein-DNA interactions. Chem Biol, 2003;10(12):1205-1213).
For additional information, contact Michael C. Fitzgerald, Department of Chemistry, Duke University, Durham, NC 27708-0346, USA. E-mail: mfitz@chem.duke.edu.
The publisher's contact information for the journal Chemistry and Biology is: Cell Press, 1100 Massachusetts Avenue, Cambridge, MA 02138, USA.
The information in this article comes under the major subject areas of Diagnostics and Proteomics.
This article was prepared by Drug Week editors from staff and other reports. Copyright 2004, Drug Week via NewsRx.com & NewsRx.net.
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