A Gene Encoding MYB-Like DNA Binding Protein, Promotes Embryogenesis in Arabidopsis
Posted on: Thursday, 10 June 2004, 06:00 CDT
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Embryogenesis is a fundamental and essential process in plant development. However, the molecular mechanisms that initiate and maintain this process are largely unclear. To gain more insight into this process, we used a chemicalinduciblc activation-tagging system (Zuo et al, 2002) to identify genes that can promote embryogenesis. Mutants were recovered in several screens. One mutant, pga37, can form embryos when cultured on a medium containing the appropriate inducer. Genomic analysis indicates that the inducible activationtagging promoter was inserted into approximately 7.8 kbp upstream of the translation-initiation codon of PGA37. Using RT- PCR, we confirmed that the activation-tagging promoter activates PGA37 in the presence of the inducer. Transgenic plants containing cDNA of PGA37 expressed from a beta-estradiol inducible promoter phenocopied phcnolypes of pga37 mutant when iduced. This further confirms that PGA37 can promote embryogenesis. Sequence analysis shows that PGA 37 encodes a MYB-like DNA binding protein, which normally functions as an activator or inhibitor to induce or repress, respectively, expression of downstream genes. Gene interaction analysis shows that PGA37 can activate the expression of LEAFY COTYLEDON 2(LEC2), a transcriplional regulator that establishes a cellular environment sufficient to initiate embryo formation. Our results suggest that PGA37 plays a key role in the initiation or maintenance of plant embryoeencsis.
Reference
Zuo, J., Niu, Q.-W., Frugis, G., and Chua, N.-H.,(2002) The WUSHEL gene promotes vegetative-to-embryonic transition in Arobidopsis. Plant 7.30, 349-359.
QI-WEN NIU1, Jianru Zuo2, and Nam-Hai Chua1,3. 1 Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021 and institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 917 Datun Road, Beijing 100101, People's Republic of China. E-mail: chua@mail.rockefeller.edu
Copyright Society for In Vitro Biology Spring 2004
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