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Isolating Effects of Microscopic Nonuniform Distributions of ^Sup 131^I on Labeled and Unlabeled Cells

Posted on: Sunday, 20 June 2004, 06:00 CDT

Radiopharmaceuticals are generally distributed nonuniformly in tissue. At the microscopic level, only a fraction of the cells in tissue are labeled. Consequently, the labeled cells receive an absorbed dose from radioactivity within the cell (self-dose) as well as an absorbed dose from radioactivity in surrounding cells (cross- dose). On the other hand, unlabeled cells only receive a cross- dose. This work uses a novel approach to examine the lethal effects of microscopic nonuniformities of ^sup 131^I individually on the labeled and unlabeled cells. Methods: A multicellular tissue model was used to investigate the lethality of microscopic nonuniform distributions of ^sup 131^I. Mammalian cells (V79) were dyed with CFDA-SE (carboxy fluorescein diacetate succinimidyl ester) and labeled with ^sup 131^I-iododeoxyuridine (^sup 131^IdU). The dyed labeled cells were then mixed with equal numbers of unlabeled cells, and 3-dimensional tissue constructs (4 10^sup 6^ cells) were formed by centrifugation in a small tube. This resulted in a uniform distribution of ^sup 131^I at the macroscopic level but nonuniform distribution at the multicellular level, wherein 50% of the cells were labeled. The multicellular clusters were maintained at 10.5C for 72 h to allow ^sup 131^I decays to accumulate. The clusters were then dismantled and the labeled (dyed) and unlabeled (undyed) cells were separately seeded for colony formation using a fluorescence- activated cell sorter. Results: The unlabeled cells, which received only a cross-dose, exhibited a mean lethal dose D^sub 37^ of 4.0 0.3 Gy. In contrast, the labeled cells received both a self-dose and a cross-dose. Isolating the effects of the self-dose resulted in a D^sub 37^ of 1.2 0.3 Gy, which was about 3.3 times more toxic per unit dose than the cross-dose. The reason for these differences appears to be primarily related to the higher relative biological effectiveness of the self-dose delivered by ^sup 131^IdU compared with the cross-dose. Theoretical modeling of the killing of labeled and unlabeled cells was achieved by considering the cellular self- doses and cross-doses. Conclusion: Cellular self-doses and cross- doses play an important role in determining the biological response of tissue to microscopic nonuniform distributions of ^sup 131^I. Prediction of the biological response requires that both self-doses and cross-doses be considered along with their relative lethality per unit dose.

Key Words: ^sup 131^I; iodine-131; iododeoxyuridine; cellular dosimetry; multicellular cluster; V79 cells; survival; ionizing radiation; [beta]-particles; radiation effects; adverse effects; therapeutic use; fluorescence-activated cell sorting

J Nucl Med 2004; 45:1050-1058

The success of diagnostic and therapeutic nuclear medicine has depended on the capacity of radiopharmaceuticals to localize in different tissues of the body. The most striking example of this is perhaps the uptake of radioiodine by the thyroid. The exquisite specificity of Na^sup 131^I in the thyroid has made this radiopharmaceutical a highly successful approach to diagnosing and treating thyroid disorders. Radiopharmaceuticals also distribute nonuniformly within organs, even down to the cellular level (1,2). This can often have a profound impact on the biological response of the tissue because the absorbed dose delivered by the emitted radiations can be similarly nonuniform, particularly for radiations having short ranges in tissue. Accordingly, correlation of biological response with absorbed dose delivered by radiopharmaceuticals has indeed been a challenge. It is apparent that the mean absorbed dose even to tissue elements, defined as a small region of tissue perhaps as small as 1 mm3, is often not an adequate variable for predicting biological response (2). Accordingly, efforts have been directed toward examining the absorbed dose at increasing levels of spatial detail even down to the cellular level (3-7). In fact, when the relative biological effectiveness (RBE) of the emitted radiations is accounted for, cellular dosimetry can be quite successful at correlating the biological response of isolated cells that contain radioactivity (8). The usefulness of cellular dosimetry at the tissue element level, often referred to as multicellular dosimetry, has yet to be validated. The problem is complex for a variety of reasons. For some radionuclides-particularly, Auger electron emitters such as ^sup 125^I and very-low-energy [beta]-emitters such as ^sup 3^H-the RBE of the emitted radiations can depend on the subcellular distribution of the radionuclide (9-12). In these instances, the dose-response arising from decays within the same cell (self-dose) may be very different than the dose-response from decays in neighboring cells (cross-dose). Furthermore, radiopharmaceuticals generally distribute nonuniformly at the multicellular level so that the cells containing radioactivity (labeled) will receive a self-dose and a cross-dose, whereas the cells that do not contain radioactivity (unlabeled) will receive only a cross-dose (5). These nuances must be accounted for in order for multicellular dosimetry to be useful for predicting biological response.

Validation of the usefulness of multicellular dosimetry for predicting biological response of tissues containing nonuniform distributions of radioactivity has been hampered by the difficulty in measuring the response of each population of cells-namely, the labeled and unlabeled cells. With the advent of high-speed fluorescence-activated cell sorting (FACS), it is now possible to sort the 2 populations for independent analysis. In this work, we have exploited FACS, in conjunction with a well-established experimental model (13-16), to separately delineate the dose- response of labeled cells and unlabeled cells in a multicellular cluster. The labeled cells contained ^sup 131^I-iododeoxyruridine (^sup 131^IdU), a radiochemical that is incorporated into the DNA of the cell nucleus. The radionuclide ^sup 131^I emits [beta]- particles with a principal mean energy of 191 keV (17), which have a corresponding range in water of approximately 400 m (78) or about 40 cell diameters. Accordingly, the labeled cells receive both self- dose and cross-dose, whereas the unlabeled cells receive cross-dose only. The responses of each population were quantified and this information was used to theoretically model the individual response of the labeled and unlabeled cells.

MATERIALS AND METHODS

Radiochemical and Quantification of Radioactivity

^sup 131^I as Na^sup 131^I was obtained from Perkin Elmer Life Sciences in 0.1N NaOH (13.6 GBq/mL). The radiochemical ^sup 131^IdU was synthesized and purified by high-performance liquid chromatography in our laboratory according to procedures previously reported (19). The ^sup 131^I activity was quantified with a Canberra HpGe (high-purity germanium) well detector (364.5-keV photopeak, photopeak efficiency = 0.33, yield = 0.812) or a Packard automatic [gamma]-counter equipped with a 7.62-cm-diameter sodium iodide well crystal (overall efficiency = 0.78 in the 260- to 470- keV energy window). The radionuclide ^sup 131^I undergoes [beta]- decay with a physical half-life of 8.04 d.

Cell Line

Cultured Chinese hamster V79 lung fibroblasts were used in this study. The different minimum essential media (MEM) used are as follows: (a) MEMA: MEM with 10% heat-inactivated (57C for 30 min) fetal calf serum (FCS), 2 mmol/E L-glutamine, 50 units/mE penicillin, and 50 g/mL streptomycin; (b) MEMB: same as MEMA except that calcium-free MEM for suspension culture was used; (c) wash MEMA: same as MEMA except FCS was replaced with fetal bovine serum; and (d) MEMC: same as MEMA except phenol red-free MEM was used. Phenol red-free medium is required for the cell sorting phase of the experiment. All media and supplements used were obtained from Life Technologies. Cells were maintained in MEMA as monolayers in Falcon (Becton Dickinson) 225-cm^sup 2^ tissue culture flasks in an incubator at 37C in 5% CO2/95% air with 100% humidified conditions. The cells were subcultured twice weekly on reaching 80%-90% confluence. The plating efficiency for these cells in MEMA, under the culture conditions described, is generally 65%. The cell line was free of mycoplasma.

Assembly of Multicellular Clusters

Multicellular clusters containing nonuniform distributions of ^sup 131^I were assembled according to protocols described in detail elsewhere (13-15). Briefly, V79 cells growing as monolayers in 225- cm^sup 2^ Falcon flasks were washed with 20 mL of phosphatebuffered saline (PBS), removed from the flask with 0.05% trypsin containing 0.53 mmol/L ethylenediaminetetraacelic acid, and suspended at 2 10^sup 6^ cells per milliliter in MEMB. Aliquots of 1 mL were placed in 2 sets of sterile 17 100 mm Falcon polypropylene round-bottom culture tubes (10 tubes in each set) and placed on a rocker-roller (Fisher Scientific) for 3-4 h in an incubator. After this conditioning period, 1 mL of MEMB containing various activities of ^sup 131^IdU (0-148 kBq/mL) was added to one set of tubes (denoted "labeled") and 1 mL of MEMB with no radioactivity was added to the second set of tubes (denoted "unlabeled"). All tubes were then returned to a rocker-roll\er kept inside the incubator. After a 14- h period of labeling with radioactivity, the tubes were removed and centrifuged at 2,000 rpm at 4C for 10 min. Aliquots of the supernatant were used to check the concentrations of radioactivity added. The remaining supernatant was decanted, the cells in both sets of tubes were washed once with 10 mL of PBS containing 50 units/ mL penicillin and 50 g/mL streptomycin (PBS-PS) and then centrifuged for 5 min at 1,000 rpm, 4C. Radiolabeled cells were loaded with a fluorescent Cell-Tracker dye (CFDA-SE [carboxy fluorescein diacetate succinimidyl ester], excitation 492 nm, emission 517 nm; Molecular Probes) by incubating the cells for 15 min at 37C in 2 mL of 10 mol/ L dye in PBS. Unlabeled cells were also treated similarly by resuspending in 2 mL PBS. All tubes were set on a rocker-roller at 37C for 15 min to allow CFDA-SE to passively diffuse into the cytoplasm of the cells where it is cleaved by intracellular esterases, becoming fluorescent. After 15 min of dye uptake, the tubes were washed once with 2 mL of prewarmed MEMA, again with 10 mL of wash MEMA, resuspended in 5 mL of MEMA, and passed through a 21- gauge needle 5 times to ensure a single-cell suspension. The cell concentration in each tube was determined with a calibrated Coulter model ZM cell counter (Coulter Electronics). The radiolabeled and subsequently dyed (2 10^sup 6^) cells were mixed with an equal number of unlabeled cells that were neither labeled with radioactivity nor dyed but were treated in the same manner as labeled cells. The pooled cells were then centrifuged at 2,000 rpm, 4C for 10 min. The supernatant was decanted, the pellets were disrupted, and the entire ~ 200-L of this final cell suspension was transferred directly to a sterile 400-L polypropylene microcentrifuge tube (Helena Plastics). The tube was washed with an additional 200 L of MEMA and the wash was transferred to the same 400-L polypropylene microcentrifuge tube. The 400-L tubes were then centrifuged at 1,000 rpm for 5 min at 4C to form multicellular clusters. The resulting clusters contained 4 10^sup 6^ cells (50% cells were radiolabeled/dyed) and weighed (7.9 0.1 mg) (16). Clusters containing 50% unlabeled cells dyed with CFDA-SE and 50% unlabeled undyed cells served as controls. The tubes containing the clusters were maintained at 10.5C. This temperature was selected because V79 cells can remain in the cluster configuration at this temperature for long periods of time (up to 72 h) without a decrease in plating efficiency. This was also true for V79 cells in suspension culture (13). The cells accumulate the preponderance of their radioactive decays while in the cluster configuration as opposed to the rudiolabeling and colony-forming periods.

Determination of Surviving Fraction of Cells in Multicellular Clusters

After 72 h at 10.5C, the supernatant was carefully removed and the tube was vortcxcd to disperse the cell cluster. The cells were rcsuspcnded in 10 mL of MEMC, transferred to 17 100 mm Falcon polypropylene tubes, washed 3 times with 10 mL of wash MHMC and resuspendcd in 2 mL of MEMC, and passed through a 21-gauge needle 5 times to disperse cells and to produce a single-cell suspension. The phenol red-free MEMC was used because phenol red interferes with the cell sorting. Aliquots were taken from each tube and the mean radioactivity per labeled cell was determined. The remaining cell suspension was transferred to Falcon 12 75 mm polystyrene tubes, which were wrapped with aluminum foil. The tubes were kept on a rocker-roller at 10.5C and transferred to the cell sorter (Becton- Dickinson FACS Vantage).

An air-cooled 488-nm argon-ion laser was used to excite the dye. The excitation and emission peaks of the fluorescent dye were 492 and 517 nm, respectively. Fluorescence in the FL-I channel was collected along with the forward angle and 90 light scatter. The histograms from the cytometer were analyzed using DiVa software (Becton-Dickinson). The flow cytometer sorted both dyepositive (labeled and dyed) and dye-negative (unlabeled and undyed) cells and separately seeded them in triplicate into Falcon 6-wcll tissue culture plates containing 4 mL of MEMA with 2 penicillin and streptomycin (100, 1,000, or 10,000 cells per well). The 6-well plates were then placed in an incubator at 37C with 5% CO2/95% air for 1 wk. The remaining unused cells were sorted into 2 tubes and subjected to flow cytometry to check the purity of sorted cells. Our experiments showed that the excitation and emission characteristics of CFDA-SE are well suited for cell sorting by FACS. Figure 1 shows the distribution of dye-positive and dye-negative cells.

FIGURE 1. Representative flow cytometric analysis of V79 cells from multicellular cluster containing 50% unlabeled cells and 50% cells labeled with 4.25 mBq per cell of ^sup 131^IdU. The cells containing ^sup 131^IdU were dyed with CFDA-SE. (A) Side scatter (SSC) vs. forward scatter (FSC) dot plot that was obtained with FACS Vantage cell sorter. Debris and aggregates were gated out as indicated by rectangle denoted P1. (B) P1-gated SSC vs. FL-1 height. (C) Number and percentage of events in each gate relative to parent gate and mean FL-1 height. (D) Distribution of undyed (P2 gate; unlabeled) and dyed (P3 gate; labeled) cells within P1 gate. Analysis indicated that, within P1 date, 46.2% of cells were undyed, 42.8% were dyed, and remaining 11% were outside P2 and P3 gates. Dyed (P3) and undyed (P2) cells were automatically and separately seeded by FACS Vantage cell sorter in known numbers into 6-well plates. Cells outside P2 and P3 gates were discarded to ensure high degree of certainty in purity of dyed and undyed populations.

The plates were removed from the incubator alter I wk and the colonies were washed 3 times with normal saline, washed 2 times with ethanol to fix. and stained with 0.05% crystal violet. The colonies were counted with a dissecting microscope (40 magnification). A colony count of 10-100 per well was considered a valid data point. The surviving fractions of labeled (dyed) and unlabeled (undyed) cells compared with parallel controls was determined for each radioactivity concentration.

RESULTS

No Migration of CFDA-SE Dye or Radioactivity

It can be seen from Figure 1 that the PACS analysis of cells from a cluster containing 50% dyed (labeled) cells and 50% undyed (unlabeled) cells after maintaining the cluster at 10.5C for 72 h showed no appreciable dye transfer from dyed to undyed cells. The dyed (labeled) and undyed (unlabeled) cells were sorted (10,000 each) into separate 12 75 mm Falcon polystyrene tubes that were assayed for ^sup 131^I activity with an HpGe detector. Figure 2 shows that the integral area under the 364-keV (^sup 131^I) photopeak was 3,145 and 20 counts for the radiolabelcd and unlabeled cells, respectively. These data provide strong evidence that there is no significant migration of ^sup 131^I radioactivity from radio- labeled cells to surrounding unlabeled cells under the experimental conditions used.

Uptake of ^sup 131^IdU in V79 Cells

The cellular uptake of ^sup 131^IdU is linearly dependent on the concentration of ^sup 131^IdU in the culture medium (MEMB). A least- squares fit of the data to a linear function gives 0.222 0.017 niBq per cell per kBq/mL. This linear dependence was also observed in previous studies with ^sup 131^IdU and ^sup 125^IdU (20,21).

FIGURE 2. [gamma]-Ray spectra show 364.5-keV photopeak of ^sup 131^I measured with HpGe well detector. Top and bottom panels show ^sup 131^I photopeaks corresponding to radioactivity in 10,000 labeled (2.4 mBq per cell) and 10,000 unlabeled cells, respectively, obtained from cell sorter. Counts were accumulated for 1,000 s. Integral counts under peaks after background subtraction were 3,145 and 20 for labeled and unlabeled cells, respectively. It can be seen clearly that unlabeled cell population contains only 0.6% as much ^sup 131^I as labeled cell population. This suggests at least a 99.4% sorting purity. Furthermore, high precision of sorting and activity measurement ensured that there was no significant migration of radioactivity from labeled cells to unlabeled cells.

Response of Multicellular Clusters to ^sup 131^IdU

Figure 3 shows the surviving fraction of cells in the multicellular cluster as a function of the 131I activity in the cluster when 50% of the cells were radiolabeled. The sorting technology allowed independent seeding of the labeled and unlabeled cells for colony formation. Therefore, separate survival curves are provided for each population. The plating efficiency for the controls was about 55% and 25% for the sorted unlabeled and labeled cells, respectively, when seeded into Falcon 6-well polystyrene flat- bottom tissue culture dishes. As indicated in previous studies (16), the plating efficiency for controls cells that undergo manual seeding into culture dishes is typically 65%. Therefore, the plating efficiency for the sorted cells appears to be affected by both the sorting process itself and the CFDA-SE dye. This is compensated for by calculating survival fractions relative to controls for dyed and undyed cells taken from control clusters containing 50% dyed cells and no radioactivity. Figure 3 shows that there are marked differences in the slopes of the survival curves for the labeled and unlabeled cells.

Cellular Absorbed Doses

Self-Absorbed Dose to Labeled Cells (Self-Dose). The mean absorbed dose to the labeled cells is delivered during 3 distinct periods of the experiment-namely, the 14-h incubation (I) period on the rocker-roller, where the radio-activity is taken up by the cells; the 72-h maintenance (M) period at 10.5C; and, finally, the 1- wk colony-forming period (CF). Accordingly, it is necessary to determine the 131I cumulated activity in the cell during each of these periods. The temporal dependence of the cellular a\ctivity and the cumulated activity are shown in Figure 4. These data are used to calculate the mean self-absorbed dose to the cell nucleus of the labeled cells as D^sub N^ = 0.32 Gy/mBq. Details of these calculations are given in the Appendix.

Cross Absorbed Dose to Labeled and Unlabeled Cells (Cross-Dose). For the medium-energy [beta]-emitter ^sup 131^I, cross-irradiation plays a major role in the absorbed dose to the labeled and unlabeled cells. The [beta]-particles have the capacity to cross-irradiate cells that reside as much as 30-40 cell diameters distant from the labeled cell during the cluster maintenance period of 72 h. The mean cross-dose to the cells is essentially the mean absorbed dose to the cluster. For ^sup 131^I, the mean absorbed dose to the cluster was determined in our previous communication to be D = 0.66 Gy/kBq of cluster activity (16). For 50% labeling, this translates to a cross- dose of 1.3 Gy/mBq in the labeled cell.

Total Absorbed Dose. The mean total absorbed dose to the labeled cell has contributions from self-doses and cross-doses, whereas the unlabeled cell only has a contribution from cross-dose. For example, a cellular uptake of 20.2 mBq per cell, the highest activity used in this study, yields a self-dose of 6.5 Gy and a cross-dose of 26.6 Gy. Hence, the ratio of self-dose to cross-dose when 50% of the cells are labeled with ^sup 131^IdU is 0.24. The labeled cells will therefore receive a total of 33.1 Gy, whereas the unlabeled cells will receive only a cross-dose equal to 26.6 Gy. It should be noted that the self-doses and cross-doses are not equally lethal per unit dose.

Dose-Response

The survival fraction versus the total absorbed dose to the labeled (self-dose + cross-dose) and unlabeled cells (cross-dose) is plotted in Figure 5. A least-squares fit of the labeled cell data to an exponential function yields a mean lethal dose D^sub 37^ of 2.9 0.3 Gy. Although there is apparently a small shoulder in the dose- response data for unlabeled cells (Fig. 5), an exponential fit has also been used to fit these data. The fit for the unlabeled cells is good (R^sup 2^ = 0.94) with a D^sub 37^ of 4.0 0.3 Gy. These values are summarized in Table 1.

DISCUSSION

To assess the effects arising out of self-doses and cross-doses delivered by ^sup 131^I, multicellular clusters were prepared by assembling clusters with equal numbers of unlabeled cells and cells radiolabeled with ^sup 131^IdU. This radiochemical, a thymidine analog, is incorporated into the DNA in the cell nucleus. It was chosen for its capacity to remain within the cell nucleus without migrating to neighboring unlabeled cells (13). This was proven in the present study with high precision, as shown in Figure 2. In our previous communication, we showed results for clusters with mixtures of labeled and unlabeled cells, wherein 100%, 10%, or 1% of the cells were radiolabeled (16). However, after disrupting the cluster, the mixtures of cells were manually seeded into dishes for colony formation. This co-seeding of labeled and unlabeled cells made it impossible to separate the dose-response of the 2 populations. In the present study, use of cell sorting technology enabled the dose- response of the labeled and unlabeled cells to be studied separately.

The exponential nature of the dose-response curve in Figure 5 for the labeled cells is characteristic of high-LET (linear energy transfer) radiations such as ?-particles (8,20), although the - particles emitted by 131I are considered to be low-LET in nature. This same type of curve was observed in our previous study when 1%, 10%, and 100% of the cells were labeled with 131IdU and the mixture of labeled and unlabeled cells was seeded for colony formation (16). A similar dose-response relationship was also observed for 131IdU in V79 cells growing in suspension (22). As discussed previously (16), there are several possible explanations for the exponential nature of the dose-response to cells labeled with ^sup 131^IdU-among them is that the damage imparted by ^sup 131^IdU is somewhat like high- LET because the decays occur within the DNA (21,23). A least- squares fit of the labeled cell data to an exponential function yields a mean lethal dose D^sub 37^ of 2.9 0.3 Gy (includes self- doses and cross-dose).

The D^sub 37^ for the unlabeled cells was 4.0 0.3 Gy. One can compare this value to our recently published results for 1% labeling in the same multicellular clusters, where the labeled and unlabeled cells were co-plated without sorting and the survival fraction of the mixed population was determined (76). In that case, the survival fractions were largely dictated by cross-irradiation of unlabeled cells and a D^sub 37^7 of 6.4 0.5 Gy was obtained. One would anticipate good agreement between the 1 % labeling data and the D^sub 37^ for unlabeled cells; however, the 2 values are considerably different. One possible reason is that the protocol for determining the dose-response of the unlabeled cells was significantly different than that for the 1% labeling case. Specifically, unlike the 1% case, the unlabeled cells were subjected to washes with PBS, treatment with the CFDA-SE dye, and ultimately passed through the cell sorter. These manipulations may have had some impact on the survival of the unlabeled cells. It is also possible that bystander effects, similar to those observed for ^sup 3^H-thymidine (13,14) and ^sup 125^IdU (75), may have played some role. This possibility is supported by the highly lethal nature of the cellular self-dose delivered by ^sup 131^IdU. Regardless of the reason, the magnitude of the responses is generally similar.

TABLE 1

Mean Lethal Doses for V79 Cells in Clusters with 50% of Cells Labeled with ^sup 131^IdU

The data in Figure 5 can be further analyzed to explicitly examine the radiotoxicity of the self-dose delivered to the labeled cells. The killing of labeled cells is the result of radiation insults inflicted by both self-doses and cross-doses, whereas the killing of unlabeled cells is caused by cross-dose alone. Therefore, assuming that there is no synergism between the self-doses and cross- doses, and that bystander effects (13-15,24) do not play a substantial role in the lethality of 131I where the cross-dose plays a major role (>4 times the self-dose), the survival fraction arising from the self-dose alone can be determined by simply dividing the survival fractions for the labeled cells by the corresponding survival fractions for the unlabeled cells (Fig. 3). This ratio is plotted as a function of both activity per labeled cell (mBq per cell) and self-dose (Gy) in Figure 6. A least-squares fit of the data to an exponential function yielded a mean lethal cellular activity A^sub 37^ = 3.7 1.0 mBq per cell that corresponds to a mean lethal self-dose D^sub 37^ = 1.2 0.3 Gy. Interestingly, the A^sub 37^ value is almost the same as the mean value of 3.6 mBq per cell for 3 experiments reported in an earlier communication that examined the radiotoxicity of ^sup 131^I in similarly handled V79 cells, except that the cells were maintained at 10.5C for 72 h as a suspension where no significant cross-dose was possible (22). This A^sub 37^ value for suspension culture also gives a D^sub 37^ of 1.2 Gy. Clearly, in the case of ^sup 131^I-IdU, the self-dose is more effective than the cross-dose in killing the V79 cells. A comparison of the D^sub 37^ values for the self-dose (1.2 Gy) with that for the cross-dose (4.0 Gy) indicates that the self-dose has an RBE of about 3.3 compared with that of the cross-dose. Furthermore, as discussed previously, the D^sub 37^ for chronic ^sup 137^Cs -[gamma]-rays is 12.2 2.4 Gy (16). Therefore, in the present work, the 131I [beta]- particles that deliver the cross-dose are about 3 times more lethal than chronic external [gamma]-rays. The sell-dose is about 10 times more lethal than the chronic [gamma]-rays. These comparisons with [gamma]-rays should be used with some caution given that the cells irradiated with [gamma]-rays were not subjected to cell sorting as in the present studies with ^sup 131^IdU.

Recall that the unlabeled cells only receive a cross-dose so Equation 2 is essentially the fit to the unlabeled cell data in Figure 5. However, Figure 7 shows the survival fraction predicted by Equation 1 (represented by the solid line) overlaid on the experimental data for the labeled cells. The fit is remarkably good, suggesting that this theoretical approach to modeling the combined effects of self-doses and cross-doses works extremely well for this radiochemical and the biological endpoint of cell killing. The success of this modeling approach augers well for ultimately modeling the response of tissues containing nonunilorm distributions of radioactivity with a multicellulardosimetry approach that takes into account both self-doses and cross-doses.

CONCLUSION

This work describes a novel methodology to assess the biological consequences of nonuniform distributions of radioactivity at the multicellular level. Cell sorting technology is used to delineate the biological effects of DNA-incorporated ^sup 131^I on both labeled and unlabeled cells in a multicellular cluster. The results clearly demonstrate that the labeled cells experience a higher degree of cytotoxicity than the unlabeled cells. Furthermore, it is clear that the RBE corresponding to the self-dose delivered by DNA- incorporated ^sup 131^I to the labeled cells is substantially higher than the RBE of the cross-dose arising from ^sup 131^I decays in the surrounding cells. Modeling the response of labeled cells to the self-doses and cross-doses clearly demonstrates the importance of assessing the self-doses and cross-doses when predicting the biological response to nonuniform distributions of ^sup 131^I.

ACKNOWLEDGMENTS

The authors thank Tammy Mui for her assistance and for operating the FACS Vantage cell sorter used to perform these e\xperiments. We are extremely grateful to Dr. Edouard I. Azzam for many stimulating discussions during the course of this work. We also thank Drs. Sonia M. de Toledo, Bogdan I. Gerashchenko, Venkatachalam Perumal, and Massimo Pinto for their excellent cooperation in our laboratory. This work was supported in part by U.S. Public Health Service (USPHS) grant R01CA83838. Finally, the authors greatly appreciate the support provided by Thomas Denny and Dana Stein at the core flow cytometry facility that is supported in part by USPHS shared instrumentation grant 1 S10 RR14753-01.

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19. Harapanhalli RS, Narra VR, Yaghmai V, et al. Vitamins as radioproleclors in vivo. II. Protection by vitamin A and soybean oil against radiation damage caused by internal radionuclides. Radiat Res. 1994;I39:115-122.

20. Howell RW, Goddu SM, Bishayee A, Rao DV. Radioprotection against lethal damage caused by chronic irradiation with radionuclides in vilro. Radial Res. 1998:150:391-399.

21. Bishayee A, Rao DV, Bouche! LG, Bolch WE, llowcll RW. Radioprotection by DMSO against cell death caused by intraccllularly locali/.cd 1-125, 1-131, and Po-210. Radial Res. 2000:153:416-427.

22. Bishayee A, Rao DV, Howell RW. Radiation protection by cysteamine against the lethal effects of intracellularly localized Auger electron, [alpha]-, and [beta]-particle emitting radionuclides. Acta Oncol. 2000;39:713-720.

23. Whaley JM, Little JB. Efficient mutation induction by 1-125 and 1-131 decays in DNA of human cells. Radiat Res. 1990; 123:68- 74.

24. Azzam EI, dc Toledo SM, Little JB. Oxidative metabolism, gap junctions and the ionizing radiation-induced bystander effect. Oncogene. 2003;22:7050-7057.

25. Makrigiorgos GM, Kassis AI, Baranowska-Kortylewicz J, et al. Radioloxicity of 5-[^sup 123^I]iodo-2'-cleoxyuridine in V79 cells: a comparison with 5-[^sup 125^I]iodo-2'-deoxyuridine. Radiat Res. 1989;! 18:532-544.

26. Howell RW, Rao DV, Saslry KSR. Macroscopic dosimetry for radioimmunotherapy: nonuniform activity distributions in solid tumors. Med Phys. 1989:16: 66-74.

Prasad V.S.V. Neti, PhD; and Roger W. Howell, PhD

Division of Radiation Research, Department of Radiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey

Received Nov. 5, 2003; revision accepted Jan. 13, 2003.

For correspondence contact: Roger W. Howell, PhD, Department of Radiology, MSB F-451, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 S. Orange Ave., Newark, NJ 07103.

E-mail: rhowell@umdnj.edu

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Copyright Society of Nuclear Medicine Jun 2004

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