Ofiling of Differentially Expressed Proteins in Normal and Malignant Breast Tissue By High Throughput Antibody Microassay - a Pilot Study
Posted on: Wednesday, 15 September 2004, 06:00 CDT
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Aims: Genes work at the protein level and, since the transcriptional activity of a gene does not necessarily reflect cellular protein expression, the identification and quantification of proteins is essential for the understanding of molecular events leading to malignant transformation.
Methods: We have employed a high-throughput protein microarray system which contains 378 well-characterized monoclonal antibodies in order to compare the gene expression pattern of malignant and adjacent normal breast tissue in a patient with primary breast cancer.
Results: We have identified a number of proteins that show increased expression levels in malignant breast tissues such as casein kinase Ie, p53, annexin XI, CDC25C, eIF-4E and MAP kinase 7. The expression of other proteins, such as the the multifunctional regulator 14-3-3[epsilon] was found to be decreased in malignant mammary tissue, whereas the majority of proteins remained unchanged when compared to the corresponding normal breast tissue. The protein expression pattern was confirmed by immunohistochemistry for 8 representative proteins known to be involved in carcinogenesis.
Conclusions: Taken together, we have described for the first time a tumor cell specificity protein expression pattern by use of a novel commercially available antibody microarray. We hypothesize that the use of protein arrays will not only increase our understanding of the molecular events but could prove useful in evaluating prognosis and in determining optimal antineoplastic therapy.
G. HUDELIST1,2, M. PACHER-ZAVISIN1, C. SINGER1, T. HOLPER3, E. KUBISTA1, M. SCHREIBER1, M. BILBAN4, K. CZERWENKA3
1 Abteilung fr Spezielle Gynkologie, UFK Wien
2 Abteilung fr Gynkologie u. Geburtshilfe, LKH Villach
3 Institut fr Klinische Pathologie, Universitt Wien
4 Institut fr Labormedizin, Universitt Wien
Copyright Urban & Fischer Verlag 2004
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