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Relation Between RNA Expression of DNA Repair Genes and Ifosfamide Chemotherapy of Melanomas in Vitro

Posted on: Wednesday, 15 September 2004, 06:00 CDT

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Aims: The active role of DNA repair genes in answer on chemotherapy of tumour patients is well known. The main aim of this study was to relate the RNA expression of DNA repair genes to chemotherapy with ifosfamide in in vitro model with 2 melanoma cell lines.

Methods: The RNA expression of DNA repair genes Ku70, Ku80, MLH1, MSH2 was evaluated applying real time PCR assays.

Results: The RNA expression of Ku70 increased 2 times 12 hours after exposition to ifosfamide. The level of MSH2 RNA-transcripts increased 4 times 18 hours after ifosfamide treatment. RNA level of MLH1 and Ku80 stayed constant. Immunohistochemically Ku70 negative cell lines (before treatment) showed firstly cytoplasmic positivity (6 hours after treatment) and afterwards (12 hours after treatment) nuclear positivity. MLH1, Ku80 and MSH2 were present in nuclei of tumour cells.

Conclusions: DNA repair genes especially Ku70 and MSH2 participate in answer of melanoma cells to chemotherapy and their RNA level changed after treatment with cytostatics.

M. KORABIOWSKA,T. SCHLOTT, P. SLOTTY, F.B. KNIG1, C. CORDON- CARDO2, U. BRINCK3

Institut fr Zytopathologie, Universitt Gttingen

1 Institut fr Neuropathologie, Universitt Gttingen,

2 Division of Molecular Pathology, Memorial Sloan Kettering Cancer Center, New York

3 Institut fr Gastroenteropathologie, Universitt Gttingen

Copyright Urban & Fischer Verlag 2004

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