May 10, 2007
Essential Oils of Piper Dumosum Rudge and Piper Aleyreanum C.DC (Piperaceae) From Brazilian Amazonian Forest
By Facundo, Valdir Alves; Ferreira, Silane Aparecida; de Morais, Selene Maia
Key Word Index
Piper dumosum, Piper aleyreanum, Piperaceae, essential oil composition, β-caryophyllene, isocaryophyllene, α-pinene, β-pinene, biclyclogermacrene, α-phellandrene.
Piper dumosum Rudge and P. aleyreanum C. DC, are known by the local population as "baburau" and "mebin nixpu," respectively.
The plants of both Piper species were collected in April 2002 near the city of Porto Velho, Rondnia, in the southeast of the Amazon forest (Brazil). A voucher specimen of each has been deposited in the Herbarium of Institute Nacional de Pesquisa da Amaznia (INPA), under number 211715 for P. dumosum and 200162 for P. aleyreanum.
Fresh leaves of P. dumosum (1.0 kg) and P. aleyreanum (1.2 kg) were steam distilled to produce essential oils in yields of 1.2% and 0.9%, respectively.
The genus Piper is distributed in tropical and subtropical regions of the world (1). Piper species are widely used in folk medicine in Latin America and the West Indies, and several chemical and biological investigations have been undertaken due to their economic and medicinal uses (2-5). Piperamides, flavonoids, lignans, aristolactams, long and short chain esters, terpenes, steroids, propenylphenols and alkaloids have been previously reported for the genus Piper (4,5). Recently, β-sitosterol, galangin and 2- methoxy-4,5-methylenedioxypropiophenone were isolated from P. aleyreanum (6). No previous reports were found in the literature for P. dumosum.
The essential oils were analyzed by a Hewlett-Packard 589OA GC and a 5973 GC/MS instrument under the following conditions: column: 5% phenyl-95% methylpolysiloxane DB-5 (J&W) fused silica capillary column (30 m x 0.25 mm x 0.25 m film thickness); carrier gas: He (1 mL/ min); injector temperature (split mode): 250C; detectortemperature (FID): 270C; column temperature: 35-180C at 3C/ min and then 180-250C at 10C/min, 15 min; mass spectra: electron impact at 70 eV (ion source at 200C). The identification of the constituents was performed by computer library search on Wiley 275, retention indices based on n-alkane series (7) and visual interpretation of the mass spectra (8-10). The list of compounds found in the oils is presented in Table I.
The authors thank the Brazilian government agencies CNPq forfinalcial suport and J. Lima, Instittito Nacional de Pesyuisa da Amaznia (INPA), Manaus, Amaznia, Brazil, for identification of plant material.
Table I. Percentage composition of the oils of P. dumosum and P. aleyreanum
1. M. Mundina, R. Vila, F. Tomi, M.P. Gupta, T. Adzet, J. Casanova and S. Canigijeral, Leaf essential oils of three Panamanian) Piper species. Phytochemistry, 47, 1277-1282(1998).
2. M.A. McFerren and E. Rodriguez, Pescicidal properties of piperovatine from Piper piscatorum (Piperacea). J. Ethnopharmacol., 75,133-139 (1987).
3. S.K. Singh, A.K. Prasad, C.E. Olsen, A. Jha, S.C. Jain, V.S. Parmar and J. Wengel, Neollgnans and alkaloids from Piper argyrophylum. Phytochemistry, 43, 1355-1360 1996.
4. V.S. Parmar, S.C. Jain, S. Gupta, S. Talwar, V.K. Rajwanshi, R. Kumar, A. Azim, S. Malhotra, N. Kumar, R. Jain, N.K. Sharma, O.D. Tyagi, SJ. Lawrie, W. Errington, O. W. Howarth, C.E. Olsen, S.K. Singh, and J. Wengel, Pofyphenols and alkaloids from Piper species. Phytochemistry, 49, 1069-1078 (1998).
5. V.S. Parmar, S.C. Jain, K S. Bisht, R. Jain, P. Taneja, A. Jha, O.D. Tuagi, A.K. Prasad, J. Wengel, C.E. Olesen and P.M. Boll, Phytochemistry of the genus Piper. Phytochemistry, 46, 597-673 (1997).
6. V.A. Facundo and S.M. Morals, Constituents of Piper aleyreanum (Piperaceae) Biochem. Syst. Ecol., 31, 111-113 (2003).
7. R.R Adams, Identification of Essential oil Components by Gas Chromatography/ Mass Spectrometry. Allured Publishing Corporation, Carol Stream, IL (1995).
8. S. Stennhagen. E. Abrahamson and F.W. McLafferty, Registry of Mass Specrral Data. John Wlley & Sons, New York, NY (1974).
9. J.W. Alencar, A.A. Craveiro, F.J.A. Mates, Kovats indexes as a preselection routine in mass spectral library search of volatiles. J. Nat. Prod., 47, 890-892(1984).
10. R.P. Adams, Identification of Essential oils by Ion Trap Mass Spactroscopy. Academic Press, San Diego, CA (1989).
Valdir Alves Facundo* and Silane Aparecida Ferreira
Departamento de Qumica da Universidade Federal de Rondnia, Av. Pres. Outra 2965, CEP 78900-500,
Porto Velho - Rondnia - Brazil
Selene Maia de Morais
Curso de Qumica da Universidade Estadual do Cear, Av. Paranjana 1700 - Campus do ltaperi, CEP 60740-000,
Fortaleza - Cear - Brazil
* Address for correspondence
Received: September 2005
Revised: May 2006
Accepted: May 2006
Copyright Allured Publishing Corporation Mar/Apr 2007
(c) 2007 Journal of Essential Oil Research : JEOR. Provided by ProQuest Information and Learning. All rights Reserved.