Holmesina is an extinct genus of pampathere, a group of armadillo-like mammals that were distantly related to modern armadillos. Fossils of members of this genus have been found mainly in Texas and Florida. Members of this genus often traveled north in the faunal interchange and were well adapted to the North American climate, like the ground sloths, glyptodonts, armadillos and other South American immigrants.

Like armadillos, the shells of Holmesina members were made up of flexible plates which allowed the animals to move more easily. These mammals were herbivores. Modern armadillos are mainly insectivores.

There are three known species in the Holmesina genus: H. septentrionalis, H. floridanus, and H. occidentalis.