Columbia launched from Kennedy Space Center on April 26. 1993 at 10:50 AM EDT and landed at Edwards AFB on May 6 at 7:29 AM PDT. The shuttle orbited 160 times at an altitude of 163 nautical miles at an inclination of 28.45 degrees and travelled 4.2 million miles. The mission lasted 9 days, 23 hours, 39 minutes, and 59 seconds.

This was a joint Spacelab mission with Germany.

88 experiments were conducted, covering materials and life sciences, technology applications, Earth observations, astronomy and atmospheric physics. Material science investigations were: Material Science Experiment Double Rack for Experiment Modules and Apparatus (MEDEA); Werkstofflabor (WL); Holographic Optics Laboratory (HOLOP); and on Unique Support Structure (USS) located aft of D-2 in cargo bay, Material Science Autonomous Payload (MAUS), and Atomic Oxygen Exposure Tray (AOET). Also located on USS were the Radiation Detectors (RD) experiments. One crystal growth experiment yielded 0.78-inch (20-mm) crystal of gallium arsenide, largest produced in space to date.

Life science research performed with Anthrorack (AR); Biolabor (BB); and Baroreflex (BA). Anthrorack, advanced mini-diagnostic laboratory, allowed most comprehensive medical screening to date of human adaptation to weightlessness. Harris, a medical doctor, set up first I.V. (intravenous) line in space, injecting Schlegel with saline as part of study to replace body fluids lost during adaptation to weightlessness. Other payload crew members also participated.

Tests with Robotics Experiment (ROTEX), an advanced robotic assembly provided by Germany, were highly successful. The ROTEX robotic arm performed first by capturing free-floating objects in space via remote control from Earth. Crew achieved two-way communications with Crew Telesupport Experiment, which featured onboard Macintosh computer to establish data link with ground control. Five crew members communicated with school children worldwide through Shuttle Amateur Radio Experiment (SAREX); Nagel also made contact with Russian cosmonauts aboard the Mir space station.

Problems encountered were an overheating orbiter refrigerator/freezer unit in middeck which forced reliance on backup to store experiment samples, and a leaking nitrogen line in wastewater tank which required an on-orbit fix. Communications with Columbia was lost for about an hour and a half on May 4 due to errant command from Mission Control in Houston. On May 2, mission managers determined enough electrical power remained to extend the flight by one day.

Columbia was crewed by Commander Steven R. Nagel, Pilot Terence T. Henricks, Mission Specialists Jerry L. Ross, Charles J. Precourt, and Bernard A. Harris, and Payload Specialists Ulrich Walter (Germany), and Hans Schlegel (Germany).