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Last updated on April 16, 2014 at 10:37 EDT

Latest Neill S. L. Wolbachia Stories

2008-07-01 06:00:15

By Fallon, Ann M Abstract In vitro production of the obligate intracellular bacterium, Wolhachia pipientis, is essential to its manipulation as a genetic tool to spread transgenes within vector populations. We have adapted the Wolbachia-infected Aa23 Aedes albopictus mosquito cell line to Eagle's minimal medium, supplemented with nonessential amino acids, glutamine, and 20% fetal bovine serum. When plated at low densities, Aa23E cells grew as patchy monolayers, comprised of non-contiguous...