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Latest Protein methods Stories

2010-09-15 17:20:41

ChIP enrichment analysis database available free online Researchers from Mount Sinai School of Medicine have developed a new computational method that will help streamline the analysis of gene expression experiments and provide scientists with a better mechanistic understanding of the differences between diseased and normal cells. The new database and software, called ChIP Enrichment Analysis (ChEA), will revolutionize how researchers identify drug targets and biomarkers. Researchers can find...

2010-08-03 14:50:28

Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme. ZFNs can be used to generate targeted genomic deletions of large segments of DNA in a wide variety of cell types and organisms. In the August issue of the journal Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc8_10.dtl), Jin-Soo Kim and colleagues...

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2010-07-28 08:55:10

Scientists in Southampton, UK, and Ulm and Karlsruhe in Germany have shown that a variant form of a fluorescent protein (FP) originally isolated from a reef coral has excellent properties as a marker protein for super-resolution microscopy in live cells. Their findings have been published online by Nature Methods and will appear in print in the upcoming August issue of that journal. Fluorescent proteins produced by a range of marine animals glow with a rainbow of colors, adding to the visual...

2010-02-01 18:39:09

Mapping DNase I hypersensitive sites has long been the standard method for identifying genetic regulatory elements such as promoters, enhancers, silencers, insulators, and locus control regions. Sequences that are nucleosome-depleted, presumably to provide access for transcription factors, are selectively digested by DNase I. Traditional low-throughput methods use Southern blots to then identify these hypersensitive sites. In the February issue of Cold Spring Harbor Protocols...

2009-12-16 12:01:59

New approach reduces number of routines needed to detect erythropoietins Austrian researchers have successfully developed a new electrophoretic method for detecting MIRCERA® and other erythropoietins (EPO) in the blood. The technique, using SARCOSYL-PAGE, has specifically enhanced sensitivity for MIRCERA, but does not alter the performance characteristics of SDS-PAGE for detecting other EPOs. Details of this study, funded by a grant from the World Anti-Doping Agency (WADA),...

2009-11-03 12:42:12

The introduction of high-throughput laboratory methods has greatly increased the pace of research into the genetics of complex diseases. Instead of focusing only on one or a few coding variants in a small sample of individuals, the ability to accurately and efficiently genotype many individuals and to cover more of the variation within individual genes has resulted in genetic studies with greater statistical power. "Laboratory Methods for High-Throughput Genotyping," from Howard Edenberg and...

2009-10-13 15:12:00

SAN DIEGO, Oct. 13 /PRNewswire/ -- SoluLinK, Inc., a leader in crosslinking products, introduces the first in a series of new, breakthrough products with the HRP-Antibody All-in-One Conjugation Kit; the first kit of its kind to allow researchers to make 100% pure conjugate probes without an HPLC. This kit employs SoluLinK's patented HydraLink(TM) crosslinking technology, which allows for 100% conversion of antibody to conjugate, and includes a unique, rapid purification column. This...

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2009-07-29 14:20:00

John Spence, a physicist at Arizona State University, is a longtime user of the Advanced Light Source at Lawrence Berkeley National Laboratory, where he has contributed to major advances in lensless imaging. It's a particularly apt propensity for someone who works with x-rays, since they can't be focused with ordinary lenses.As new light sources evolve to produce brighter x-rays in faster pulses, lensless imaging becomes ever more critical for science. Among the promises of superbright,...

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2009-07-21 09:39:42

Scientists at the U.S. Department of Energy's (DOE) Lawrence Berkeley National Laboratory have developed a fast and efficient way to determine the structure of proteins, shortening a process that often takes years into a matter of days. The high-throughput protein pipeline could allow scientists to expedite the development of biofuels, decipher how extremophiles thrive in conditions that kill most organisms, and better understand how proteins carry out life's vital functions. The technique...

2009-06-23 07:30:00

Additions to the Human Protein Atlas, available exclusively through Sigma-Aldrich, bring the portfolio to over 6,100 antibodies powered by Atlas Antibodies ST. LOUIS, June 23 /PRNewswire-FirstCall/ -- Sigma-Aldrich (Nasdaq: SIAL) today announced the release of over 2,300 antibody additions to its Prestige Antibodies(R) powered by Atlas Antibodies product line (www.sigma.com/prestige), bringing the total number of highly validated antibodies to over 6,100. Developed by the Human Proteome...


Word of the Day
malpais
  • The ragged surface of a lava-flow.
'Malpais' translates from Spanish as 'bad land.'