September 11, 2012
Ants Have The Best Noses In The Insect Kingdom
John Neumann for redOrbit.com — Your Universe Online
Researchers have long known that ants live in some of the most complex social structures of any animal. With the division of labor and communication between individual ants and the ability to solve complex problems, the reason for such complex structures may be the increased number of smells that ants can discern.
A team of scientists, led by Lawrence Zwiebel and his research team at Vanderbilt, recently completed the first full map of an ant´s olfactory system, discovering that they have four to five times more odor receptors than most other insects. This provides ants with their sense of taste and smell, writes David Salisbury in a statement.
They found the industrious insects have genes that make about 400 distinct odorant receptors, which are special proteins that detect different odors. By comparison, silk moths have 52 odorant receptors, fruit flies have 61, mosquitoes range from 74 to 158 and the honeybee has 174.
“The most exciting moment for me was when the analysis came back showing that we had identified more than 400 OR genes, the largest number of any known insect species,” said Xiaofan Zhou, the research associate who headed up the characterization process.
“It meant that we had successfully taken the first step toward gaining a new level of understanding of the complex social system that has made ants one of the most successful families on the planet.”
For some time, scientists have also known that chemical communication plays an important role in ant behavior. “So it´s a reasonable supposition that this dramatic expansion in odor-sensing capability is what allowed ants to develop such a high level of social organization,” said Zwiebel, in the Aug. 30 issue of the journal PLoS Genetics.
The olfactory system of most insects is centered in their antennae and is broadly made up of three different classes of receptors: odorant receptors (ORs), that identify different aromatic compounds and pheromones; gustatory receptors (GRs), that distinguish between different tastes and react to some pheromones; and newly discovered ionotropic glutamate receptors (IRs), that are narrowly tuned to various poisonous and toxic compounds.
The study found that the primary expansion in the ant´s olfactory system is focused on ORs. The number of GRs and IRs are comparable to those found in other insects. The initial automated analysis of the two ant genomes found only about 100 genes for ORs and ten GRs.
“We knew these numbers were low because olfactory receptors are very difficult to identify,” said Xiaofan Zhou, the research associate who headed up the characterization process.
Zhou and his colleagues had to design a novel automated bioinformatic process for this purpose, combined with extensive manual evaluation.
The researchers also compared both the identity and expression levels of ORs in the two species and found significant differences. This wasn´t surprising because the two species were selected to reflect the high level of diversity that exists within the ant family.
Carpenter ants live in large colonies with long-lived queens that produce all the fertilized eggs. There are two castes of sterile workers. When the queen dies so does the colony. Jumping ants, by contrast, live in small groups. The difference between the queen and workers is limited, and some workers can mate and lay fertilized eggs.
“The differences in receptors are most probably associated with the difference in lifestyles of the two species,” Zhou said.
Similarly, their analysis found important differences in the ORs in the antennae of males and females. Overall, they found that the males have only one third the number of ORs that the females express. “The primary role of males is fertilization of eggs, so we assume that the ORs that males express and that females don´t are probably tuned to pheromones produced by the queen,” Zhou said.
The team also took the initial steps in identifying the chemical signals that set off specific ORs. Research Associate Jesse Slone adapted an assay that the group initially developed for matching ORs with chemical signals in the malaria mosquito. The assay involves inserting the gene for a receptor into frog eggs so that the receptors are expressed on the egg´s surface. By wiring the eggs and then exposing them to different chemical compounds, the eggs produce a measurable electric signal when the receptor is activated.
Slone used this assay to identify the compounds that trigger one OR on each of the ant species. He found that an aromatic compound found in anise triggered the OR from the jumping ant that he tested. The receptors were found in both males and workers. Since studies have shown that anise oil has a repellent and/or insecticidal effect on some species of insects, the compound may be a general insect repellent that this OR is designed to detect, Slone speculates.
“This is just the beginning. But we have demonstrated that we have the basic tools we need to act as ℠OR detectives´ to map the ants ℠odor space´ and identify the chemical signals that trigger specific behaviors in the ant´s extensive repertoire,” Slone said.
This represents the opening of a major new research avenue for the Zwiebel Lab, which has been focused on pioneering work deconstructing the olfactory system of the malaria mosquito. “When I was in graduate school, a group of us dreamed about deciphering the role that genes might play in ant social behavior. So I couldn´t pass up this opportunity when it came along."
“It´s taken 30 years but we´ve finally gotten to the point where we can actually do these studies,” Slone said.